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Literature summary extracted from

  • van Straaten, K.E.; Gonzalez, C.F.; Valladares, R.B.; Xu, X.; Savchenko, A.V.; Sanders, D.A.
    The structure of a putative S-formylglutathione hydrolase from Agrobacterium tumefaciens (2009), Protein Sci., 18, 2196-2202.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.12
-
 Agrobacterium tumefaciens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.12 by the sitting drop-vapor diffusion technique, at 2 A resolution. Crystals belong to space group P1 with unit cell dimensions a = 66.43 A, b = 68.95 A, c = 95.65 A, alpha = 92.41, beta = 99.79, gamma = 98.43. Contacts between the two monomers in the dimer are formed both by hydrogen bonds and salt bridges Agrobacterium tumefaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.12 C54S shows a 4fold decrease in alpha-naphthyl butyrate activity. Is insensitive to the inhibitor N-ethylmaleimide Agrobacterium tumefaciens
3.1.2.12 S147A is unable to hydrolyse alpha-naphthyl butyrate Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.12 N-ethylmaleimide binding of the inhibitor molecule to the conserved cysteine residue Cys54 located in the active site of the enzyme, consequently, blocking the binding of substrate to the active site Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.12 0.02
-
 4-nitrophenyl caproate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 0.05
-
 4-nitrophenyl propionate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 0.11
-
 4-nitrophenyl butyrate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 0.35
-
 alpha-naphthyl propionate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 0.44
-
 naphthalen-1-yl n-butanoate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 0.48
-
 4-nitrophenyl acetate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.12 Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.12
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.12 4-nitrophenyl acetate + H2O
-
 Agrobacterium tumefaciens 4-nitrophenol + acetate
-
 ?
3.1.2.12 4-nitrophenyl butyrate + H2O highest activity Agrobacterium tumefaciens 4-nitrophenol + butyrate
-
 ?
3.1.2.12 4-nitrophenyl caproate + H2O
-
 Agrobacterium tumefaciens 4-nitrophenol + caproate
-
 ?
3.1.2.12 4-nitrophenyl propionate + H2O
-
 Agrobacterium tumefaciens 4-nitrophenol + propionate
-
 ?
3.1.2.12 alpha-naphthyl butyrate + H2O
-
 Agrobacterium tumefaciens alpha-naphthol + butyrate
-
 ?
3.1.2.12 alpha-naphthyl propionate + H2O
-
 Agrobacterium tumefaciens alpha-naphthol + propionate
-
 ?
3.1.2.12 additional information is unable to hydrolyse alpha-naphthyl dodecanoate, 4-nitrophenyl caprate, 4-nitrophenyl laurate, and 4-nitrophenyl palmitate. Potential role for Cys54 in regulation of enzyme activity through S-glutathionylation. Hydrolyzes C-O bonds with high affinity toward short to medium chain esters (C3-C4). Has more affinity toward C3 and C4 4-nitrophenyl esters than toward C3 and C4 alpha-naphthyl esters Agrobacterium tumefaciens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.12 homodimer crystallography Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
3.1.2.12 Atu1476
-
 Agrobacterium tumefaciens
3.1.2.12 S-formylglutathione hydrolase
-
 Agrobacterium tumefaciens
3.1.2.12 SFGH
-
 Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.12 0.67
-
 4-nitrophenyl caproate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 1.73
-
 naphthalen-1-yl n-butanoate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 1.84
-
 4-nitrophenyl propionate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 1.9
-
 alpha-naphthyl propionate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 1.95
-
 4-nitrophenyl acetate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 4.48
-
 4-nitrophenyl butyrate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.12 3.93
-
 naphthalen-1-yl n-butanoate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 4.07
-
 4-nitrophenyl acetate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 5.54
-
 alpha-naphthyl propionate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 38.5
-
 4-nitrophenyl propionate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 39.6
-
 4-nitrophenyl caproate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens
3.1.2.12 40.8
-
 4-nitrophenyl butyrate in Tris-HCl buffer, pH 8.0 Agrobacterium tumefaciens