EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.133 | Ca2+ | the enzyme is activated by 15% at 5 mM | Parageobacillus caldoxylosilyticus | |
3.2.1.133 | Mn2+ | the enzyme is activated by 51% at 5 mM | Parageobacillus caldoxylosilyticus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.133 | gene GcaTK4MA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression of His6-tagged TK4MA in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM101 | Parageobacillus caldoxylosilyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.133 | Co2+ | the enzyme is inhibited by 23% at 5 mM | Parageobacillus caldoxylosilyticus | |
3.2.1.133 | Cu2+ | the enzyme is inhibited by 15% at 5 mM | Parageobacillus caldoxylosilyticus | |
3.2.1.133 | Fe2+ | the enzyme is inhibited by 28% at 5 mM | Parageobacillus caldoxylosilyticus | |
3.2.1.133 | Li+ | the enzyme is inhibited by 67% at 5 mM | Parageobacillus caldoxylosilyticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.133 | additional information | the enzyme sequence contains no signal sequence | Parageobacillus caldoxylosilyticus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.133 | additional information | 5 mM Zn2+ has no effect on the enzyme activity | Parageobacillus caldoxylosilyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 70030 | - |
x * 70030, sequence calculation | Parageobacillus caldoxylosilyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.133 | Parageobacillus caldoxylosilyticus | C0LZ63 | gene GcaTK4MA; gene GcaTK4MA | - |
3.2.1.133 | Parageobacillus caldoxylosilyticus TK4 | C0LZ63 | gene GcaTK4MA; gene GcaTK4MA | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.133 | beta-cyclodextrin + H2O | - |
Parageobacillus caldoxylosilyticus | ? | - |
? | |
3.2.1.133 | beta-cyclodextrin + H2O | - |
Parageobacillus caldoxylosilyticus TK4 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.133 | ? | x * 70030, sequence calculation | Parageobacillus caldoxylosilyticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.133 | MAase | - |
Parageobacillus caldoxylosilyticus |
3.2.1.133 | maltogenic amylase | - |
Parageobacillus caldoxylosilyticus |
3.2.1.133 | More | the enzyme belongs to a subclass of cyclodextrin-hydrolyzing enzymes, belonging to glycoside hydrolase family 13, GH13 | Parageobacillus caldoxylosilyticus |
3.2.1.133 | TK4MA | - |
Parageobacillus caldoxylosilyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 50 | - |
- |
Parageobacillus caldoxylosilyticus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 30 | 80 | profile, overview | Parageobacillus caldoxylosilyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 7 | - |
- |
Parageobacillus caldoxylosilyticus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.133 | 6 | 9 | activity range, profile, overview | Parageobacillus caldoxylosilyticus |