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Literature summary extracted from

  • Zhang, T.; Gong, F.; Peng, Y.; Chi, Z.
    Optimization for high-level expression of the Pichia guilliermondii recombinant inulinase in Pichia pastoris and characterization of the recombinant inulinase (2009), Process Biochem., 44, 1335-1339.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.7 synthesis high-level expression in Pichia pastoris leads to production of enzyme at 286.8 U/ml and 8873 U/mg Meyerozyma guilliermondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.7 expression in Pichia pastoris Meyerozyma guilliermondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.7 Ag+ 1 mM, 20% residual activity Meyerozyma guilliermondii
3.2.1.7 Hg2+ 1 mM, 25% residual activity Meyerozyma guilliermondii
3.2.1.7 Mg2+ 1 mM, 80% residual activity Meyerozyma guilliermondii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.7 Ca2+ 1 mM, 35% activation Meyerozyma guilliermondii
3.2.1.7 Co2+ 1 mM, 20% activation Meyerozyma guilliermondii
3.2.1.7 Cu2+ 1 mM, 30% activation Meyerozyma guilliermondii
3.2.1.7 Fe2+ 1 mM, 35% activation Meyerozyma guilliermondii
3.2.1.7 Fe3+ 1 mM, 20% activation Meyerozyma guilliermondii
3.2.1.7 K+ 1 mM, 35% activation Meyerozyma guilliermondii
3.2.1.7 Li+ 1 mM, 35% activation Meyerozyma guilliermondii
3.2.1.7 Mn2+ 1 mM, 35% activation Meyerozyma guilliermondii
3.2.1.7 Na+ 1 mM, 35% activation Meyerozyma guilliermondii
3.2.1.7 Zn2+ 1 mM, 40% activation Meyerozyma guilliermondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.7 57600
-
 x * 57600, SDS-PAGE Meyerozyma guilliermondii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Meyerozyma guilliermondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7 recombinant enzyme Meyerozyma guilliermondii

Subunits

EC Number Subunits Comment Organism
3.2.1.7 ? x * 57600, SDS-PAGE Meyerozyma guilliermondii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 60
-
-
 Meyerozyma guilliermondii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.7 60
-
 stable up to, rapid inactivation above Meyerozyma guilliermondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 6
-
-
 Meyerozyma guilliermondii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.7 6 7
-
 Meyerozyma guilliermondii