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Literature summary extracted from

  • Park, I.S.; Lin, C.H.; Walsh, C.T.
    Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase (1997), Proc. Natl. Acad. Sci. USA, 94, 10040-10044.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.35 expression in Escherichia coli Enterococcus casseliflavus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.35 1.8
-
 D-serine pH 7.5 Enterococcus casseliflavus
6.3.2.35 1.9
-
 ATP pH 7.5 Enterococcus casseliflavus
6.3.2.35 7.2
-
 D-asparagine pH 7.5 Enterococcus casseliflavus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.35 Enterococcus casseliflavus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.35 recombinant protein Enterococcus casseliflavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.35 D-alanine + D-2-aminobutanoate + ATP
-
 Enterococcus casseliflavus D-alanyl-D-2-aminobutanoate + ADP + phosphate
-
 ?
6.3.2.35 D-alanine + D-alanine + ATP formation of D-alanyl-D-serine is 400fold prefered over formation of D-alanyl-D-alanine Enterococcus casseliflavus D-alanyl-D-alanine + ADP + phosphate
-
 ?
6.3.2.35 D-alanine + D-allo-threonine + ATP
-
 Enterococcus casseliflavus D-alanyl-D-allo-threonine + ADP + phosphate
-
 ?
6.3.2.35 D-alanine + D-asparagine + ATP
-
 Enterococcus casseliflavus D-alanyl-D-asparagine + ADP + phosphate
-
 ?
6.3.2.35 D-alanine + D-glutamine + ATP
-
 Enterococcus casseliflavus D-alanyl-D-glutamine + ADP + phosphate
-
 ?
6.3.2.35 D-alanine + D-homoserine + ATP
-
 Enterococcus casseliflavus D-alanyl-D-homoserine + ADP + phosphate
-
 ?
6.3.2.35 D-alanine + D-serine + ATP formation of D-alanyl-D-serine is 400fold prefered over formation of D-alanyl-D-alanine Enterococcus casseliflavus D-alanyl-D-serine + ADP + phosphate
-
 ?
6.3.2.35 D-alanine + D-threonine + ATP
-
 Enterococcus casseliflavus D-alanyl-D-threonine + ADP + phosphate
-
 ?
6.3.2.35 additional information amino acid preference in decreasing order: D-Ser, D-Asn, D-Thr, D-Gln, D-homoserine, D-aminobutanoate, D-allo-Thr Enterococcus casseliflavus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.35 VanC2
-
 Enterococcus casseliflavus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.35 8.17
-
 D-serine pH 7.5 Enterococcus casseliflavus
6.3.2.35 8.87
-
 D-asparagine pH 7.5 Enterococcus casseliflavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.35 8 9.5 broad Enterococcus casseliflavus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.2.35 1.23
-
 D-asparagine pH 7.5 Enterococcus casseliflavus
6.3.2.35 4.5
-
 D-serine pH 7.5 Enterococcus casseliflavus