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Literature summary extracted from

  • Sun, Y.; Fang, Y.; Yoon, M.S.; Zhang, C.; Roccio, M.; Zwartkruis, F.J.; Armstrong, M.; Brown, H.A.; Chen, J.
    Phospholipase D1 is an effector of Rheb in the mTOR pathway (2008), Proc. Natl. Acad. Sci. USA, 105, 8286-8291.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 additional information the overexpression of tuberous sclerosis complex 2, TSC2, suppresses PLD1 activation, whereas the knockdown or deletion of TSC2 leads to elevated basal activity of PLD Homo sapiens
3.1.4.4 Rheb Rheb binds and activates PLD1 in vitro in a GTP-dependent manner. Overexpression of the small GTPase Rheb activates PLD1 in cells by about 2.5fold in the absence of mitogenic stimulation, and the knockdown of Rheb impairs serum stimulation of PLD activation, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 coexpression of HA-tagged PLD1 and various epitope-tagged GTPases in HEK-293 cells for interaction analysis, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.4 additional information knockdown of isozyme PLD2 by two independent shRNAs has no effect on Rheb activation of S6K1 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 1-butanol
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+
-
 Homo sapiens
3.1.4.4 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O Homo sapiens
-
 choline + phosphatidic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 HEK-293 cell isozymes PLD1 and PLD2 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O
-
 Homo sapiens choline + phosphatidic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D1
-
 Homo sapiens
3.1.4.4 PLD
-
 Homo sapiens
3.1.4.4 PLD1
-
 Homo sapiens
3.1.4.4 PLD2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.4.4 physiological function phospholipase D and its product phosphatidic acid are upstream regulators of the mitogenic mTOR signaling in both mitogenesis and the mechanical stimulation of skeletal muscle growth. Isozyme PLD1, but not PLD2, is required for Rheb activation of the mTOR pathway and for Rheb activation of S6K1, PLD1 is a bona fide effector for Rheb Homo sapiens