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Literature summary extracted from

  • Galpin, J.; Clemens, S.; Kermode, A.
    The carboxy-terminal ER-retention motif, SEKDEL, influences the N-linked glycosylation of recombinant human ?-l-iduronidase but has little effect on enzyme activity in seeds of Brassica napus and Nicotiana tabacum (2010), Plant Sci., 178, 440-447.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.76 expression of IDUA with and without a C-terminal endoplasmic reticulum-retention sequence SEKDEL, in seeds of Brassica napus and Nicotiana tabacum under the control of regulatory 5'-, signal-peptide-encoding-, and 3'-sequences from the arcelin 5-I gene of Phaseolus vulgaris Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.76 76600
-
 x * 76600, recombinant glycosylated enzyme expressed from Brassica napus seeds, SDS-PAGE, x * 76700, recombinant glycosylated enzyme expressed from Nicotiana tabacum seeds, SDS-PAGE Homo sapiens
3.2.1.76 76700
-
 x * 76600, recombinant glycosylated enzyme expressed from Brassica napus seeds, SDS-PAGE, x * 76700, recombinant glycosylated enzyme expressed from Nicotiana tabacum seeds, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.76 Homo sapiens
-
 gene IDUA
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.76 glycoprotein the enzyme is N-glycosylated. The carboxy-terminal endoplasmic reticulum-retention motif, SEKDEL, influences the N-linked glycosylation of the recombinant enzyme Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.76 recombinant enzyme with and without a C-terminal ER-retention signal from seeds of Brassica napus and Nicotiana tabacum by lectinb affinity, immunoaffinity, and adsorption chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.76 32
-
 purified recombinant enzyme expressed from Brassica napus seeds Homo sapiens
3.2.1.76 39.7
-
 purified recombinant enzyme expressed from Nicotiana tabacum seeds Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.76 4-methylumbelliferyl-alpha-L-iduronoside + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-iduronic acid
-
 ?
3.2.1.76 additional information substrate specificity of recombinant enzyme with and without a C-terminal ER-retention signal from seeds of Brassica napus and Nicotiana tabacum, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.76 ? x * 76600, recombinant glycosylated enzyme expressed from Brassica napus seeds, SDS-PAGE, x * 76700, recombinant glycosylated enzyme expressed from Nicotiana tabacum seeds, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.76 alpha-L-iduronidase
-
 Homo sapiens
3.2.1.76 IDUA
-
 Homo sapiens