Literature summary extracted from
Wang, M.; Li, D.; Sun, X.; Zhu, Y.; Nong, H.; Zhang, J.
Characterization of a root-specific ?-thioglucoside glucohydrolase gene in Carica papaya and its recombinant protein expressed in Pichia pastoris (2009), Plant Sci., 177, 716-723.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.2.1.147 |
ascorbate |
stimulates 9fold at 0.2 mM, maximally 18fold at 00.8 mM |
Carica papaya |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.147 |
gene pTGG2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Pichia pastoris |
Carica papaya |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.147 |
NaCl |
inhibits, completely at 1 M |
Carica papaya |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.147 |
2.24 |
- |
sinigrin |
pH 6.0, 37°C |
Carica papaya |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.147 |
59500 |
- |
x * 70000, recombinant TGG2, SDS-PAGE, x * 59500, about, TGG2 sequence calculation |
Carica papaya |
3.2.1.147 |
70000 |
- |
x * 70000, recombinant TGG2, SDS-PAGE, x * 59500, about, TGG2 sequence calculation |
Carica papaya |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.147 |
Carica papaya |
C9WCQ1 |
cv. Solo II, gene pTGG2 |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.2.1.147 |
glycoprotein |
CpTGG2 may have 5 N-glycosylation sites |
Carica papaya |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.147 |
recombinant TGG2 from Pichia pastoris |
Carica papaya |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.2.1.147 |
root |
CpTGG2 is specifically expressed in the root |
Carica papaya |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.2.1.147 |
24.3 |
- |
purified recombinant enzyme |
Carica papaya |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.147 |
sinigrin + H2O |
i.e. 2-propenyl glucosinolate, substrate saturation at 6 mM |
Carica papaya |
allyl isothiocyanate + D-glucose + ? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.147 |
? |
x * 70000, recombinant TGG2, SDS-PAGE, x * 59500, about, TGG2 sequence calculation |
Carica papaya |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.147 |
beta-thioglucoside glucohydrolase |
- |
Carica papaya |
3.2.1.147 |
More |
TGGs belong to the glycosyl hydrolase family 1 |
Carica papaya |
3.2.1.147 |
myrosinase |
- |
Carica papaya |
3.2.1.147 |
TGG2 |
- |
Carica papaya |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.147 |
40 |
- |
- |
Carica papaya |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.2.1.147 |
10 |
90 |
CpTGG2 is active in broad temperature range, peaks at 40°C, gradually decreases between 40°C and 90°C, and shows 13% residual activity at 80°C and 1% at 90°C |
Carica papaya |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.147 |
80 |
- |
purified recombinant TGG2, 20 min, 50% activity remaining |
Carica papaya |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.147 |
28.4 |
- |
sinigrin |
pH 6.0, 37°C |
Carica papaya |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.147 |
7.5 |
- |
- |
Carica papaya |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.2.1.147 |
5 |
11 |
CpTGG2 is active in broad pH range, almost inactive below pH 3.0 and above pH 13.0 |
Carica papaya |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
3.2.1.147 |
Carica papaya |
TGG2 sequence calculation |
- |
6.35 |
kcat/KM [mM/s]
EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.147 |
13 |
- |
sinigrin |
pH 6.0, 37°C |
Carica papaya |
|