EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.195 | expression in Escherichia coli | Arabidopsis thaliana |
2.3.1.196 | expression in Escherichia coli | Clarkia breweri |
2.3.1.196 | expression in Escherichia coli | Nicotiana tabacum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.196 | Ca2+ | 5 mM, strong inhibition | Clarkia breweri | |
2.3.1.196 | Co2+ | 5 mM, strong inhibition | Clarkia breweri | |
2.3.1.196 | Cu2+ | 5 mM, strong inhibition | Clarkia breweri | |
2.3.1.196 | Mg2+ | 5 mM, strong inhibition | Clarkia breweri | |
2.3.1.196 | Zn2+ | 5 mM, strong inhibition | Clarkia breweri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.195 | 0.0105 | - |
acetyl-CoA | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
2.3.1.195 | 0.165 | - |
(3Z)-hex-3-en-ol | pH 7.7, 25°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
2.3.1.196 | 0.019 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Nicotiana tabacum | |
2.3.1.196 | 0.0205 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
2.3.1.196 | 0.035 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Nicotiana tabacum | |
2.3.1.196 | 0.0468 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
2.3.1.196 | 0.0978 | - |
cinnamyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
2.3.1.196 | 0.464 | - |
cinnamoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
2.3.1.196 | 0.818 | - |
acetyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.195 | 50290 | - |
calculated from sequence | Arabidopsis thaliana |
2.3.1.196 | 47000 | 49500 | gel filtration | Clarkia breweri |
2.3.1.196 | 50650 | - |
calculated from sequence | Clarkia breweri |
2.3.1.196 | 51010 | - |
calculated from sequence | Nicotiana tabacum |
2.3.1.196 | 51014 | - |
x * 51014, calculated from sequence | Nicotiana tabacum |
2.3.1.196 | 55000 | - |
1 * 55000, SDS-PAGE | Clarkia breweri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.195 | acetyl-CoA + (3Z)-hex-3-en-ol | Arabidopsis thaliana | - |
(Z)-3-hexen-1-yl acetate + coenzyme A | - |
? | |
2.3.1.196 | benzoyl-CoA + benzyl alcohol | Clarkia breweri | the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma | benzyl benzoate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.195 | Arabidopsis thaliana | Q9SRQ2 | - |
- |
2.3.1.196 | Clarkia breweri | Q8GT21 | - |
- |
2.3.1.196 | Nicotiana tabacum | Q8GT20 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.196 | complete, non-fusion BEBT enzyme from the crude Escherichia coli extract | Clarkia breweri |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.196 | flower | the BEBT gene is expressed in different parts of the flowers with maximal RNA transcript levels in the stigma | Clarkia breweri | - |
2.3.1.196 | leaf | induced upon wounding | Nicotiana tabacum | - |
2.3.1.196 | leaf | no expression in the leaves under normal conditions. BEBT expression is induced in damaged leaves, reaching a maximum 6 h after damage occurres | Clarkia breweri | - |
2.3.1.196 | petal | 30% of the activity in stigma | Clarkia breweri | - |
2.3.1.196 | sepal | 30% of the activity in stigma | Clarkia breweri | - |
2.3.1.196 | stamen | 30% of the activity in stigma | Clarkia breweri | - |
2.3.1.196 | stigma | the BEBT gene is expressed in different parts of the flowers with maximal RNA transcript levels in the stigma | Clarkia breweri | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.195 | acetyl-CoA + (3Z)-hex-3-en-ol | - |
Arabidopsis thaliana | (3Z)-hex-3-en-1-yl acetate + CoA | - |
? | |
2.3.1.195 | acetyl-CoA + (3Z)-hex-3-en-ol | - |
Arabidopsis thaliana | (Z)-3-hexen-1-yl acetate + coenzyme A | - |
? | |
2.3.1.196 | acetyl-CoA + benzyl alcohol | the Km-value for acetyl-CoA (0.818 mM) strongly suggests that acetyl-CoA is not commonly used by BEBT as the acyl donor in vivo | Clarkia breweri | benzyl acetate + CoA | - |
? | |
2.3.1.196 | benzoyl-CoA + benzyl alcohol | - |
Clarkia breweri | benzyl benzoate + CoA | - |
? | |
2.3.1.196 | benzoyl-CoA + benzyl alcohol | - |
Nicotiana tabacum | benzyl benzoate + CoA | - |
? | |
2.3.1.196 | benzoyl-CoA + benzyl alcohol | the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma | Clarkia breweri | benzyl benzoate + CoA | - |
? | |
2.3.1.196 | benzoyl-CoA + cinnamyl alcohol | - |
Clarkia breweri | cinnamyl benzoate + CoA | - |
? | |
2.3.1.196 | cinnamoyl-CoA + benzyl alcohol | the Km-value for cinnamoyl-CoA (0.464 mM) strongly suggests that acetyl-CoA is not commonly used by BEBT as the acyl donor in vivo | Clarkia breweri | ? + CoA | - |
? | |
2.3.1.196 | additional information | coumaroyl-CoA does not act as substrate | Clarkia breweri | ? | - |
? | |
2.3.1.196 | additional information | no activity with acetyl-CoA at concentrations below 1 mM | Nicotiana tabacum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.195 | ? | x * 50290, calculated from sequence | Arabidopsis thaliana |
2.3.1.196 | ? | x * 51014, calculated from sequence | Nicotiana tabacum |
2.3.1.196 | monomer | 1 * 55000, SDS-PAGE | Clarkia breweri |
2.3.1.196 | monomer | 1 * 50650, calculated from sequence | Clarkia breweri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.195 | ChAT | - |
Arabidopsis thaliana |
2.3.1.196 | BEBT | - |
Clarkia breweri |
2.3.1.196 | benzoyl-CoA:benzyl alcohol benzoyl transferase | - |
Clarkia breweri |
2.3.1.196 | benzoyl-coenzyme A:benzyl alcohol benzoyl transferase | - |
Clarkia breweri |
2.3.1.196 | HSR201 | - |
Nicotiana tabacum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.195 | 22 | - |
assay at | Arabidopsis thaliana |
2.3.1.196 | 22 | - |
assay at | Clarkia breweri |
2.3.1.196 | 22 | - |
assay at | Nicotiana tabacum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.196 | 30 | - |
30 min, stable | Clarkia breweri |
2.3.1.196 | 37 | - |
30 min, 20% loss of activity | Clarkia breweri |
2.3.1.196 | 50 | - |
30 min, complete inactivation | Clarkia breweri |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.196 | 0.9 | - |
cinnamoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
2.3.1.196 | 21.5 | - |
acetyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
2.3.1.196 | 47.2 | - |
cinnamyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
2.3.1.196 | 85.2 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
2.3.1.196 | 124 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.195 | 7.7 | - |
- |
Arabidopsis thaliana |
2.3.1.196 | 7.7 | - |
- |
Clarkia breweri |
2.3.1.196 | 7.7 | - |
assay at | Nicotiana tabacum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.196 | 6.5 | 9 | pH 6.5: 39% of maximal activity, pH 9.0: 69% of maximal activity | Clarkia breweri |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.196 | physiological function | the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma | Clarkia breweri |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.196 | 0.4 | - |
cinnamoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
2.3.1.196 | 2.5 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
2.3.1.196 | 4 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
2.3.1.196 | 4.6 | - |
cinnamyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
2.3.1.196 | 17.5 | - |
acetyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri |