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Literature summary extracted from

  • Pleskot, R.; Potocky, M.; Pejchar, P.; Linek, J.; Bezvoda, R.; Martinec, J.; Valentova, O.; Novotna, Z.; Zarsky, V.
    Mutual regulation of plant phospholipase D and the actin cytoskeleton (2010), Plant J., 62, 494-507.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.4 additional information antisense-mediated knockdown of tobacco pollen PLDs results in inhibition of pollen tube growth Nicotiana tabacum
3.1.4.4 N232A site-directed mutagenesis of isozyme PLDbeta1, leads to reduced actin and G-protein binding Nicotiana tabacum
3.1.4.4 N323A/T382A site-directed mutagenesis of isozyme PLDbeta1, leads to reduced actin and G-protein binding Nicotiana tabacum
3.1.4.4 T382A site-directed mutagenesis of isozyme PLDbeta1, leads to reduced actin and G-protein binding Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 additional information no inhibition by tert-butanol; no inhibition by tert-butanol; no inhibition by tert-butanol Nicotiana tabacum
3.1.4.4 n-butanol PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes; PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes; PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 additional information Nicotiana tabacum ability of PLD-generated phosphatidic acid to control actin polymerization and the reciprocal ability of actin to specifically modulate PIP2-dependent PLD, PLDbeta, activity through direct interaction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Nicotiana tabacum D7P5D9 cv. Samsun and Bright Yellow 2, different isozymes
-
3.1.4.4 Nicotiana tabacum D7P5E0 cv. Samsun and Bright Yellow 2, isozyme PLDbeta1
-
3.1.4.4 Nicotiana tabacum D7P5E1 cv. Samsun and Bright Yellow 2, isozyme PLDdelta
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 cell culture from cv. Bright Yellow 2 Nicotiana tabacum
-
3.1.4.4 additional information semi-quantitative PCR analysis of PLD expression and tissue distribvution Nicotiana tabacum
-
3.1.4.4 pollen from cv. Samsun Nicotiana tabacum
-
3.1.4.4 pollen tube
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information ability of PLD-generated phosphatidic acid to control actin polymerization and the reciprocal ability of actin to specifically modulate PIP2-dependent PLD, PLDbeta, activity through direct interaction Nicotiana tabacum ?
-
 ?
3.1.4.4 additional information enhanced binding of isozyme PLDbeta1 to actin is mediated by amino acid residues Asn323 and Thr382 Nicotiana tabacum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 PIP2-independent PLD
-
 Nicotiana tabacum
3.1.4.4 PLD
-
 Nicotiana tabacum
3.1.4.4 PLDalpha
-
 Nicotiana tabacum
3.1.4.4 PLDalpha3
-
 Nicotiana tabacum
3.1.4.4 PLDbeta
-
 Nicotiana tabacum
3.1.4.4 PLDbeta1
-
 Nicotiana tabacum
3.1.4.4 PLDdelta
-
 Nicotiana tabacum

General Information

EC Number General Information Comment Organism
3.1.4.4 malfunction PLD inhibition disrupts the actin cytoskeleton in tobacco pollen tubes, with severe disorganization of actin, both in the apex and in the shank Nicotiana tabacum
3.1.4.4 physiological function PLD activity controls the structure of the actin cytoskeleton in tobacco pollen tubes, actin forms affect differently the activity of distinct tobacco C2 PLDs, overview Nicotiana tabacum