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Literature summary extracted from

  • Crowell, D.N.; Huizinga, D.H.; Deem, A.K.; Trobaugh, C.; Denton, R.; Sen, S.E.
    Arabidopsis thaliana plants possess a specific farnesylcysteine lyase that is involved in detoxification and recycling of farnesylcysteine (2007), Plant J., 50, 839-847.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.5 gene FCLY, encoded on chromosome 5, DNAS and amino acid sequence determination and analysis Arabidopsis thaliana
1.8.3.6 gene FCLY, encoded on chromosome 5, DNA and amino acid sequence determination and analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.8.3.5 additional information T-DNA insertions mutants fcly-1 and fcly-2 plants exhibit reduced FCLY mRNA levels, as determined by semi-quantitative RT-PCR, and reduced FC lyase activity compared with Columbia (Col-0) and wild-type siblings. Significantly, germination of fcly-1 and fcly-2 seeds is inhibited by exogenous ABA at concentrations that did not affect wild-type Col-0 seeds Arabidopsis thaliana
1.8.3.6 additional information T-DNA insertions mutants fcly-1 and fcly-2 plants exhibit reduced FCLY mRNA levels, as determined by semi-quantitative RT-PCR, and reduced FC lyase activity compared with Columbia (Col-0) and wild-type siblings. Significantly, germination of fcly-1 and fcly-2 seeds is inhibited by exogenous ABA at concentrations that did not affect wild-type Col-0 seeds Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.6 additional information S-geranyl-L-cysteine or S-geranylgeranyl-L-cysteine fail to inhibit S-farnesyl-L-cysteine lyase Arabidopsis thaliana
1.8.3.6 N-acetyl-S-farnesyl-L-cysteine a 50fold molar excess of unlabeled N-acetyl-S-farnesyl-L-cysteine inhibits the conversion of [1-3H]-S-farnesyl-L-cysteine to [1-3H]-farnesal by more than 50% Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.3.5 membrane
-
 Arabidopsis thaliana 16020
-
1.8.3.6 membrane
-
 Arabidopsis thaliana 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.3.5 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O Arabidopsis thaliana the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.5 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O Arabidopsis thaliana Col-0 the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.6 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O Arabidopsis thaliana the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.6 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O Arabidopsis thaliana Col-0 the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.5 Arabidopsis thaliana P57681 gene FCLY
-
1.8.3.5 Arabidopsis thaliana Col-0 P57681 gene FCLY
-
1.8.3.6 Arabidopsis thaliana P57681
-
-
1.8.3.6 Arabidopsis thaliana P57681 gene FCLY
-
1.8.3.6 Arabidopsis thaliana Col-0 P57681 gene FCLY
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.3.5 additional information the enzyme is ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-
1.8.3.6 flower ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-
1.8.3.6 leaf ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-
1.8.3.6 additional information the enzyme is ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-
1.8.3.6 root ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-
1.8.3.6 seedling ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-
1.8.3.6 stem ubiquitously expressed in Arabidopsis tissues and organs Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.5 N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
 ?
1.8.3.5 N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana Col-0 (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
 ?
1.8.3.5 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.5 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana Col-0 (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.6 N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
 ?
1.8.3.6 N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana Col-0 (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
 ?
1.8.3.6 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme is specific for S-farnesyl-L-cysteine. No activity towards S-geranyl-L-cysteine or S-geranylgeranyl-L-cysteine Arabidopsis thaliana (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.6 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.6 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme is specific for S-farnesyl-L-cysteine. No activity towards S-geranyl-L-cysteine or S-geranylgeranyl-L-cysteine Arabidopsis thaliana Col-0 (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?
1.8.3.6 S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine Arabidopsis thaliana Col-0 (2E,6E)-farnesal + L-cysteine + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.3.5 FC lyase
-
 Arabidopsis thaliana
1.8.3.5 FCLY
-
 Arabidopsis thaliana
1.8.3.6 At5g63910
-
 Arabidopsis thaliana
1.8.3.6 FC lyase
-
 Arabidopsis thaliana
1.8.3.6 FCLY
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.5 30
-
 assay at Arabidopsis thaliana
1.8.3.6 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.5 7.5
-
 assay at Arabidopsis thaliana
1.8.3.6 7.4
-
 assay at Arabidopsis thaliana
1.8.3.6 7.5
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.8.3.5 malfunction T-DNA insertions into the FCLY gene cause significant decreases in FC lyase activity and an enhanced response to abscisic acid in seed germination assays. The effects of FCLY mutations on abscisic acidsensitivity are even greater in the presence of exogenous S-(2E,6E)-farnesyl-L-cysteine Arabidopsis thaliana
1.8.3.5 metabolism the enzyme is involved in the salvage cycle for S-(2E,6E)-farnesyl diphosphate in plants, overview Arabidopsis thaliana
1.8.3.5 physiological function Arabidopsis FC lyase recognizes amide-linked S-(2E,6E)-farnesyl-L-cysteine and may have a role in deprenylation of farnesylated proteins Arabidopsis thaliana
1.8.3.6 malfunction T-DNA insertions into the FCLY gene cause significant decreases in FC lyase activity and an enhanced response to abscisic acid in seed germination assays. The effects of FCLY mutations on abscisic acid sensitivity are even greater in the presence of exogenous farnesyl-L-cysteine Arabidopsis thaliana
1.8.3.6 metabolism the enzyme is involved in the salvage cycle for S-(2E,6E)-farnesyl diphosphate in plants, overview Arabidopsis thaliana
1.8.3.6 physiological function Arabidopsis FC lyase recognizes amide-linked S-(2E,6E)-farnesyl-L-cysteine and may have a role in deprenylation of farnesylated proteins Arabidopsis thaliana