Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fernandez-Alvarez, A.; Elias-Villalobos, A.; Ibeas, J.I.
    The O-mannosyltransferase PMT4 is essential for normal appressorium formation and penetration in Ustilago maydis (2009), Plant Cell, 21, 3397-3412.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.109 agriculture Ustilago maydis enzyme homologs are not found in plants and may be a new target for fungal control strategies, research into mechanisms of fungal plant penetration Ustilago maydis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.109 PCR-amplification to create mutants, full gene transfer via expression vector to Schizosaccharomyces pombe (deficient in pmt) for complementation studies Ustilago maydis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.109 additional information complementation studies with Schizosaccharomyces pombe deletion mutants show that pmt1 is a functional homolog Ustilago maydis
2.4.1.109 additional information complementation studies with Schizosaccharomyces pombe deletion mutants show that pmt4 is a functional homolog Ustilago maydis
2.4.1.109 additional information pmt2 deletion mutants are not viable Ustilago maydis
2.4.1.109 additional information pmt4 and pmt1 and double deletion mutants are viable with normal growth rates and mating, normal disease symptoms are developed in infected Zea mays Ustilago maydis
2.4.1.109 additional information the single deletion mutant of pmt1 is viable with normal growth rates and mating, causes disease development in Zea may comparable to wild-type, no increased sensitivity to antifungal substances such as congo red, chlorpromazine, calcofluor white, and caffein or to thermal stress (34 and 36°C), oxidative stress (H2O2), osmotic stress (SDS and sorbitol), or salt-based stress (NaCl, CaCl2) Ustilago maydis
2.4.1.109 additional information the single deletion mutant of pmt4 is viable with normal growth rates and mating, causes no disease symptoms in Zea mays, not even in plants infected via the stigma, no increased sensitivity to antifungal substances such as congo red, chlorpromazine, calcofluor white, and caffein or to thermal stress (34 and 36°C), to oxidative stress (H2O2), to osmotic stress by sorbitol but to SDS, or to salt-based stress (NaCl, CaCl2), no changed activity of secreted hydrolytic enzymes (cellulase, pectinase, amylase activity), normal filament formation but lower appressorium formation and aberrant appressorium formation, hyphae are built close to the plant surface but do not intrude the epidermal cell layer, inhibition of the defensive plant reactive oxygen species formation does not restore virulence Ustilago maydis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Ustilago maydis
-
 maize smut fungus causes tumor formation in Zea mays, strains FB1, FB2 strains, AB33, and AB34
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 O-mannosyltransferase
-
 Ustilago maydis
2.4.1.109 PMT1
-
 Ustilago maydis
2.4.1.109 PMT2
-
 Ustilago maydis
2.4.1.109 PMT4
-
 Ustilago maydis
2.4.1.109 Um05433
-
 Ustilago maydis
2.4.1.109 Um10749
-
 Ustilago maydis
2.4.1.109 Um11220
-
 Ustilago maydis

General Information

EC Number General Information Comment Organism
2.4.1.109 malfunction pmt4 deletion mutant with strong reduction in appressorium formation, the few formed appressoria lack the capacity to penetrate the plant cuticle, reduced pathogenicity, no effects on vegetative growth or mating, normal polar growth of infectious hyphae Ustilago maydis
2.4.1.109 physiological function pmt4 is important for pathogenesis (appressorium formation and penetration) Ustilago maydis