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Literature summary extracted from

  • Andersson, D.; Chakrabarty, R.; Bejai, S.; Zhang, J.; Rask, L.; Meijer, J.
    Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties (2009), Phytochemistry, 70, 1345-1354.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 ascorbic acid the myrosinase isozymes show different activation–inhibition responses towards ascorbic acid with maximal activity around 0.7-1 mM Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.147 overexpression of His-tagged root isozymes TGG4 and TGG5 and His-tagged leaf isozyme TGG1 in Pichia pastoris Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.045
-
 sinigrin pH 5.5, isozyme TGG1 Arabidopsis thaliana
3.2.1.147 0.245
-
 sinigrin pH 5.5, isozyme TGG4 Arabidopsis thaliana
3.2.1.147 0.547
-
 sinigrin pH 5.5, isozyme TGG5 Arabidopsis thaliana
3.2.1.147 28
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG4 Arabidopsis thaliana
3.2.1.147 34
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG1 Arabidopsis thaliana
3.2.1.147 80
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG5 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.147 additional information the isozymes are active in a wide range of salt concentrations but sensitive to high salt concentrations Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Arabidopsis thaliana
-
 six myrosinase genes, TGG1-TGG6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147 recombinant His-tagged root isozymes TGG4 and TGG5 and His-tagged leaf isozyme TGG1 from Pichia pastoris by nickel affinity chromatography to homogeneity Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 leaf isozymne TGG1 Arabidopsis thaliana
-
3.2.1.147 root isozymnes TGG4 and TGG5 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Arabidopsis thaliana 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.147 additional information differences in basal activity of myrosinase isozymes, no activity with desulfosinigrin Arabidopsis thaliana ?
-
 ?
3.2.1.147 sinigrin + H2O i.e. 2-propenyl glucosinolate Arabidopsis thaliana allyl isothiocyanate + D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.147 myrosinase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.147 40
-
 isozyme TGG1 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.147 20 60 isozyme TGG1, 65% of maximal activity at 20°C, progressive increase that peaks at 40°C, high activity at 50°C, inactivation above 60°C Arabidopsis thaliana
3.2.1.147 20 80 20% of maximal activity at 20°C, progressive increase that peaks at 70°C, 30% of maximal activity at 80°C Arabidopsis thaliana
3.2.1.147 20 90 20% of maximal activity at 20°C, progressive increase that peaks at 60°C, 5% of maximal activity at 90°C Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.147 additional information
-
 the isozymes are rather thermostable Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.147 1.2
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG1 Arabidopsis thaliana
3.2.1.147 2.3
-
 sinigrin pH 5.5, isozyme TGG1 Arabidopsis thaliana
3.2.1.147 7.3
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG4 Arabidopsis thaliana
3.2.1.147 12
-
 sinigrin pH 5.5, isozyme TGG4 Arabidopsis thaliana
3.2.1.147 17
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG5 Arabidopsis thaliana
3.2.1.147 46
-
 sinigrin pH 5.5, isozyme TGG5 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 5.5
-
 isozyme TGG5 Arabidopsis thaliana
3.2.1.147 5.5 10.5 isozymes TGG1 and TGG4 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.147 2.5 12 isozymes TGG4 and TGG5 are active at pH 2.5, and TGG4 shows a broad optimum at pH 5.5-10.5, while TGG5 peaks at pH 5.5 and than loses activity at pH 6.5 and 8.5, both show 50% of maximal activity at pH 11.5 Arabidopsis thaliana
3.2.1.147 4.5 12 isozyme TGG1, inactive below pH 4.5, rather unaffected by differences in pH above 5.5 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.147 0.035
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG1 Arabidopsis thaliana
3.2.1.147 0.21
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG5 Arabidopsis thaliana
3.2.1.147 0.26
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.5, isozyme TGG4 Arabidopsis thaliana
3.2.1.147 48
-
 sinigrin pH 5.5, isozyme TGG4 Arabidopsis thaliana
3.2.1.147 53
-
 sinigrin pH 5.5, isozyme TGG1 Arabidopsis thaliana
3.2.1.147 85
-
 sinigrin pH 5.5, isozyme TGG5 Arabidopsis thaliana