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Literature summary extracted from

  • Jia, H.; Terkawi, M.A.; Aboge, G.O.; Goo, Y.K.; Luo, Y.; Li, Y.; Yamagishi, J.; Nishikawa, Y.; Igarashi, I.; Sugimoto, C.; Fujisaki, K.; Xuan, X.
    Characterization of a leucine aminopeptidase of Babesia gibsoni (2009), Parasitology, 136, 945-952.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of GST-tagged LAP in Escherichia coli DH12S cells Babesia gibsoni

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 bestatin
-
 Babesia gibsoni
3.4.11.1 Ca2+
-
 Babesia gibsoni
3.4.11.1 Cu2+
-
 Babesia gibsoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.05
-
 L-proline-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 0.095
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 0.102
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 0.108
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 0.109
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, native enzyme Babesia gibsoni
3.4.11.1 0.189
-
 L-arginine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ activates moderately Babesia gibsoni
3.4.11.1 Mg2+ activates slightly Babesia gibsoni
3.4.11.1 Mn2+ activates, best divalent cation Babesia gibsoni
3.4.11.1 additional information the enzyme is strongly dependent on metal divalent cations Babesia gibsoni
3.4.11.1 Ni2+ activates slightly Babesia gibsoni
3.4.11.1 Zn2+ activates slightly Babesia gibsoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 57000
-
 x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE Babesia gibsoni
3.4.11.1 85000
-
 x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE Babesia gibsoni

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Babesia gibsoni C5NSV0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 merozoite
-
 Babesia gibsoni
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-alanine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-alanine + 7-amino-4-methylcoumarine
-
 ?
3.4.11.1 L-arginine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-arginine + 7-amino-4-methylcoumarine
-
 ?
3.4.11.1 L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-leucine + 7-amino-4-methylcoumarine
-
 ?
3.4.11.1 L-phenylalanine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-phenylalanine + 7-amino-4-methylcoumarine
-
 ?
3.4.11.1 L-proline-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-proline + 7-amino-4-methylcoumarine
-
 ?
3.4.11.1 additional information substrate specificity, overview Babesia gibsoni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE Babesia gibsoni

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Babesia gibsoni
3.4.11.1 leucine aminopeptidase
-
 Babesia gibsoni
3.4.11.1 M17 leucyl aminopeptidase
-
 Babesia gibsoni
3.4.11.1 More the enzyme belongs to the M17 family leucine aminopeptidases Babesia gibsoni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
-
 Babesia gibsoni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 2.03
-
 L-proline-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 8.2
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 24.61
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 37.79
-
 L-arginine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 198.8
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
-
 Babesia gibsoni

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 7 10 activity range Babesia gibsoni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.1 0.011
-
 L-proline-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 0.086
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 0.227
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 0.752
-
 L-arginine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
3.4.11.1 1.942
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni