BRENDA - Enzyme Database

Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu(CAA)

Brzezicha, B.; Schmidt, M.; Makalowska, I.; Jarmolowski, A.; Pienkowska, J.; Szweykowska-Kulinska, Z.; Nucleic Acids Res. 34, 6034-6043 (2006)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.1.1.203
medicine
this methyltransferase is a novel downstream Myctarget which mediates Myc-induced cell proliferation and growth. Therefore, the characterization of hTrm4 substrate specificity can be essential since this enzyme is a potential target for cancer therapies
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.202
-
Saccharomyces cerevisiae
2.1.1.203
expression of the human Trm4 (hTrm4) cDNA in yeast partially complements the lack of the endogenous Trm4p enzyme
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.203
nucleolus
-
Homo sapiens
5730
-
2.1.1.203
nucleoplasm
-
Homo sapiens
5654
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.203
86471
-
x * 86471, calculated from sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.203
S-adenosyl-L-methionine + cytosine34 in tRNA
Homo sapiens
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.202
Saccharomyces cerevisiae
P38205
-
-
2.1.1.203
Homo sapiens
Q08J23
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.1.1.203
S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
the formation of 5-methylcytosine34 is a strictly intron-dependent process
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA precursor
yeast pre-tRNALeu
710135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
yeast tRNASer(AGE)
710135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
yeast tRNAAsp(GUC)
710135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
2.1.1.203
S-adenosyl-L-methionine + cytosine34 in tRNA
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)
710135
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
-
?
2.1.1.203
S-adenosyl-L-methionine + cytosine34 in tRNA precursor
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA). The hTrm4 MTase has a much narrower specificity against the yeast substrates than its yeast orthologue. The human enzyme is not able to form 5-methylcytosine34 at positions 48 and 49 of human and yeast tRNA precursors. Intron in the human pre-tRNALeu(CAA) is indispensable for the C5-methylation of cytosine in the first position of the anticodon. The modification of C34 depends on the nucleotide sequence surrounding the position to be modified and on the structure of intron-containing prolongated anticodon stem
710135
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.203
?
x * 86471, calculated from sequence
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.202
30
-
assay at
Saccharomyces cerevisiae
2.1.1.203
30
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.202
7.5
-
assay at
Saccharomyces cerevisiae
2.1.1.203
7.5
-
assay at
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.1.1.203
medicine
this methyltransferase is a novel downstream Myctarget which mediates Myc-induced cell proliferation and growth. Therefore, the characterization of hTrm4 substrate specificity can be essential since this enzyme is a potential target for cancer therapies
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.202
-
Saccharomyces cerevisiae
2.1.1.203
expression of the human Trm4 (hTrm4) cDNA in yeast partially complements the lack of the endogenous Trm4p enzyme
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.203
nucleolus
-
Homo sapiens
5730
-
2.1.1.203
nucleoplasm
-
Homo sapiens
5654
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.203
86471
-
x * 86471, calculated from sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.203
S-adenosyl-L-methionine + cytosine34 in tRNA
Homo sapiens
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.202
S-adenosyl-L-methionine + cytosine34 in tRNA precursor
yeast pre-tRNALeu
710135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine48 in tRNA
yeast tRNASer(AGE)
710135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
2.1.1.202
S-adenosyl-L-methionine + cytosine49 in tRNA
yeast tRNAAsp(GUC)
710135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
2.1.1.203
S-adenosyl-L-methionine + cytosine34 in tRNA
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)
710135
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
-
?
2.1.1.203
S-adenosyl-L-methionine + cytosine34 in tRNA precursor
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA). The hTrm4 MTase has a much narrower specificity against the yeast substrates than its yeast orthologue. The human enzyme is not able to form 5-methylcytosine34 at positions 48 and 49 of human and yeast tRNA precursors. Intron in the human pre-tRNALeu(CAA) is indispensable for the C5-methylation of cytosine in the first position of the anticodon. The modification of C34 depends on the nucleotide sequence surrounding the position to be modified and on the structure of intron-containing prolongated anticodon stem
710135
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.203
?
x * 86471, calculated from sequence
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.202
30
-
assay at
Saccharomyces cerevisiae
2.1.1.203
30
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.202
7.5
-
assay at
Saccharomyces cerevisiae
2.1.1.203
7.5
-
assay at
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.1.1.203
physiological function
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.203
physiological function
specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA)
Homo sapiens