Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hultman, K.; Bjoerklund, U.; Hansson, E.; Jern, C.
    Potentiating effect of endothelial cells on astrocytic plasminogen activator inhibitor type-1 gene expression in an in vitro model of the blood-brain barrier (2010), Neuroscience, 166, 408-415.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.68 plasminogen activator inhibitor type-1 major inhibitor of tissue-type plasminogen activator in the blood Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.68 plasminogen + H2O Rattus norvegicus
-
 plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.68 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.68 astroglial cell primary astroglial cultures prepared from newborn rat cerebral cortices Rattus norvegicus
-
3.4.21.68 cell culture in vitro model of the blood-brain barrier, primary rat astrocyte-enriched cultures are co-cultured with primary adult rat brain microvascular endothelial cells on opposite sides of a transwell membrane Rattus norvegicus
-
3.4.21.68 endothelial cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.68 plasminogen + H2O
-
 Rattus norvegicus plasmin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.68 t-PA
-
 Rattus norvegicus
3.4.21.68 Tissue-type plasminogen activator
-
 Rattus norvegicus

Expression

EC Number Organism Comment Expression
3.4.21.68 Rattus norvegicus influence of endothelial cells on astrocytic gene expression of t-PA using an in vitro model of the blood-brain barrier, primary rat astrocyte-enriched cultures are cocultured with primary adult rat brain microvascular endothelial cells on opposite sides of a transwell membrane, after co-culturing for 9-11 days, the cultures are treated with lipopolysaccharide for 8 h or 24 h, t-PA mRNA expression in astrocytes are unaffected by co-cultivation and/or lipopolysaccharide treatment, analysis of endothelial t-PA gene expression reveals increased unaltered t-PA mRNA levels additional information
3.4.21.68 Rattus norvegicus the effects of lipopolysaccharide treatment on the astrocyte levels t-PA transcripts are investigated, lipopolysaccharide does not induce any significant alterations in t-PA gene expression in either monocultured or co-cultured astrocytes additional information
3.4.21.68 Rattus norvegicus the influence of astrocytes on endothelial t-PA mRNA expression is studied, for co-cultured endothelial cells, no altered t-PA mRNA levels are observed between the co-cultured cells and the monocultured endothelial cells after treatment with lipopolysaccharide additional information
3.4.21.68 Rattus norvegicus when astrocytes are co-cultured with endothelial cells, no significant alterations in the mRNA levels of t-PA mRNA expression are observed in cocultured astrocytes additional information

General Information

EC Number General Information Comment Organism
3.4.21.68 metabolism tissue-type plasminogen activator functions as the main activator of the fibrinolytic process in the intravascular compartment Rattus norvegicus
3.4.21.68 physiological function tissue-type plasminogen activator is a serine protease that converts plasminogen into the active enzyme plasmin, which in turn degrades the fibrin of the forming thrombus Rattus norvegicus