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Literature summary extracted from

  • Wang, J.; Okamoto, Y.; Tsuboi, K.; Ueda, N.
    The stimulatory effect of phosphatidylethanolamine on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD) (2007), Neuropharmacology, 54, 8-15.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.54 1,2-dioleoylphosphatidylcholine 0.1 mM, 1.3fold stimulation Rattus norvegicus
3.1.4.54 1,2-dioleoylphosphatidylethanolamine 0.1 mM, increases activity with N-palmitoylphosphatidylamine (0.025 mM) 3.3fold, increases activity with N-arachidonoylphosphatidylamine (0.025 mM), N-oleoylphosphatidylamine (0.025 mM) and N-stearoylphosphatidylamine (0.025 mM), 2.6-2.7fold Rattus norvegicus
3.1.4.54 1,2-dipalmitoylphosphatidylcholine 0.1 mM, 1.3fold stimulation Rattus norvegicus
3.1.4.54 1,2-dipalmitoylphosphatidylethanolamine
-
 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.54 expression in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.54 Cd2+ 10 mM, more than 90% inhibition Rattus norvegicus
3.1.4.54 Cu2+ 10 mM, more than 90% inhibition Rattus norvegicus
3.1.4.54 Hg2+ 10 mM, more than 90% inhibition Rattus norvegicus
3.1.4.54 Zn2+ 10 mM, more than 90% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.54 0.0017
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dioleoyl-phosphatidylethanolamine Rattus norvegicus
3.1.4.54 0.0022
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA Rattus norvegicus
3.1.4.54 0.0038
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dipalmitoyl-phosphatidylcholine Rattus norvegicus
3.1.4.54 0.004
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2 Rattus norvegicus
3.1.4.54 0.0069
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dioleoyl-phosphatidylserine Rattus norvegicus
3.1.4.54 0.0116
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dioleoyl-phosphatidylethanolamine Rattus norvegicus
3.1.4.54 0.0122
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dipalmitoyl-phosphatidylcholine Rattus norvegicus
3.1.4.54 0.0122
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dioleoyl-phosphatidylserine Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.54 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.54 Ba2+ 10 mM, stimulates Rattus norvegicus
3.1.4.54 Ca2+ in the presence of 100 mM phospholipid (phosphatidylethanolamine, phosphatidylcholine, or phosphatidylserine), Ca2+ dose-dependently activates NAPE-PLD in varied degrees. Ca2+ at 10 mM stimulates the activity 2.1 fold in the presence of phosphatidylethanolamine. In the presence of phosphatidylcholine or phosphatidylserine, the stimulatory effects of 10 mM Ca2+ (5.2 and 10 fold, respectively) are similar to the effect by 10 mM Ca2+ in the absence of phospholipids (7fold) Rattus norvegicus
3.1.4.54 Co2+ 10 mM, stimulates Rattus norvegicus
3.1.4.54 Mg2+ 10 mM, stimulates Rattus norvegicus
3.1.4.54 Ni2+ 10 mM, stimulates Rattus norvegicus
3.1.4.54 Sr2+ 10 mM, stimulates Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.54 Rattus norvegicus Q769K2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.54
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.54 N-arachidonoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-arachidonoylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-oleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-oleoylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-palmitoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-palmitoylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-stearoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-stearoylethanolamine + phosphatidate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.54 NAPE-PLD
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.54 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.54 7.4
-
 assay at Rattus norvegicus