Literature summary extracted from

Flint, J.; Taylor, E.; Yang, M.; Bolam, D.N.; Tailford, L.E.; Martinez-Fleites, C.; Dodson, E.J.; Davis, B.G.; Gilbert, H.J.; Davies, G.J.
Structural dissection and high-throughput screening of mannosylglycerate synthase (2005), Nat. Struct. Mol. Biol., 12, 608-614.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.4.1.269	hanging-drop method	Rhodothermus marinus

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.4.1.269	D100A	inactive mutant enzyme	Rhodothermus marinus
2.4.1.269	D102A	inactive mutant enzyme	Rhodothermus marinus
2.4.1.269	H217A	inactive mutant enzyme	Rhodothermus marinus
2.4.1.269	R131A	inactive mutant enzyme	Rhodothermus marinus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.4.1.269	EDTA	-	Rhodothermus marinus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.1.269	21.3	-	D-lactate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
2.4.1.269	23.7	-	glycolic acid	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
2.4.1.269	81.2	-	GDP-mannose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
2.4.1.269	89.4	-	GDP-mannose	pH 7.8, 65°C, cosubstrate: D-glycerate	Rhodothermus marinus
2.4.1.269	96.5	-	D-glycerate	pH 7.8, 65°C, cosubstrate: GDP-mannose	Rhodothermus marinus
2.4.1.269	121.9	-	D-glycerate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
2.4.1.269	124.9	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
2.4.1.269	138.6	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.4.1.269	Mg2+	absolute requirement for divalent metal ions. 200 mM of Mg2+, Ca2+, Mn2+, Ni2+ and Co2+ giving relative rates of 1.0, 0.6, 0.33, 0.16 and 0.15, respectively	Rhodothermus marinus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.1.269	GDP-alpha-D-glucose + D-glycerate	Rhodothermus marinus	the enzyme catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate	2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.269	Rhodothermus marinus	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.269	GDP-alpha-D-glucose + D-glycerate	the enzyme catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate	Rhodothermus marinus	2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP	-	?
2.4.1.269	GDP-alpha-D-glucose + D-glycerate	at 37°C mannosylglycerate synthase shows donor flexibility and can use alpha-GDP-D-Man, alpha-GDP-D-Glc, beta-GDP-L-fucose, alpha-UDP-D-Man and alpha-UDP-D-Glc, but not Man-1-P, as substrates. The kcat/KM-value for GDP-glucose is 2.5fold lower than the value for GDP-mannose at 25°C. The kcat/KM-value for GDP-glucose is 8.6fold lower than the value for GDP-mannose at 65°C	Rhodothermus marinus	2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP	-	?
2.4.1.269	GDP-mannose + D-glycerate	at 37°C mannosylglycerate synthase shows donor flexibility and can use alpha-GDP-D-Man, alpha-GDP-D-Glc, beta-GDP-L-fucose, alpha-UDP-D-Man and alpha-UDP-D-Glc, but not Man-1-P, as substrates. The kcat/KM-value for GDP-glucose is 2.5fold lower than the value for GDP-mannose at 25°C. The kcat/KM-value for GDP-glucose is 8.6fold lower than the value for GDP-mannose at 65°C	Rhodothermus marinus	2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP	-	?
2.4.1.269	GDP-mannose + D-lactate	the kcat/KM-value for D-lactate is 70fold lower than the value for D-glycerate at 25°C	Rhodothermus marinus	?	-	?
2.4.1.269	GDP-mannose + glycolic acid	the kcat/KM-value for glycolic acid is 70fold lower than the value for glycolic acid at 25°C	Rhodothermus marinus	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.1.269	MGS	-	Rhodothermus marinus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.4.1.269	25	-	assay at	Rhodothermus marinus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.4.1.269	0.0026	-	D-lactate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
2.4.1.269	0.0052	-	glycolic acid	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
2.4.1.269	0.63	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
2.4.1.269	1.02	-	GDP-mannose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
2.4.1.269	1.02	-	D-glycerate	pH 7.8, 25°C, cosubstrate: GDP-mannose	Rhodothermus marinus
2.4.1.269	1.1	-	GDP-glucose	pH 7.8, 25°C, cosubstrate: D-glycerate	Rhodothermus marinus
2.4.1.269	6.1	-	GDP-mannose	pH 7.8, 65°C, cosubstrate: D-glycerate	Rhodothermus marinus
2.4.1.269	6.5	-	D-glycerate	pH 7.8, 65°C, cosubstrate: GDP-mannose	Rhodothermus marinus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.4.1.269	7.8	-	assay at	Rhodothermus marinus

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Release 2023.1 (January 2023)