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Literature summary extracted from

  • Empadinhas, N.; Pereira, P.J.; Albuquerque, L.; Costa, J.; Sá-Moura, B.; Marques, A.T.; Macedo-Ribeiro, S.; da Costa, M.S.
    Functional and structural characterization of a novel mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus reveals its dual substrate specificity (2011), Mol. Microbiol., 79, 76-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.217 corresponding gene is expressed in Escherichia coli Rubrobacter xylanophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.217 crystal structure of the unliganded MpgS and of its complexes with the nucleotide and sugar donors, at 2.2, 2.8 and 2.5 A resolution respectively Rubrobacter xylanophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.217 A164S/A208S/F211Y MpgS triple mutant engineered to reinforce the hydrogen-bonding network Rubrobacter xylanophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.217 0.26
-
 GDP-mannose wild-type Rubrobacter xylanophilus
2.4.1.217 0.34
-
 3-phospho-D-glycerate wild-type, with GDP-mannose Rubrobacter xylanophilus
2.4.1.217 0.49
-
 GDP-mannose A164S/A208S/F211Y mutant Rubrobacter xylanophilus
2.4.1.217 0.81
-
 3-phospho-D-glycerate A164S/A208S/F211Y mutant, with GDP-mannose Rubrobacter xylanophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.217 Co2+ effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ Rubrobacter xylanophilus
2.4.1.217 Mg2+ strictly dependent on, effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ Rubrobacter xylanophilus
2.4.1.217 Mn2+ effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ Rubrobacter xylanophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.217 Rubrobacter xylanophilus B7SY86 DSM 9941
-
2.4.1.266 Rubrobacter xylanophilus DSM 9941
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.217 purified from cell extracts Rubrobacter xylanophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.217 0.0232
-
 with GDP-mannose as a substrate Rubrobacter xylanophilus
2.4.1.266 0.0629
-
 with GDP-glucose as a substrate Rubrobacter xylanophilus DSM 9941

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate
-
 Rubrobacter xylanophilus GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
 Rubrobacter xylanophilus DSM 9941 GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.217 mannosyl-3-phosphoglycerate synthase
-
 Rubrobacter xylanophilus
2.4.1.217 mannosyl-3-phosphoglycerate synthase/phosphatase
-
 Rubrobacter xylanophilus
2.4.1.217 MPGS GT81 family Rubrobacter xylanophilus
2.4.1.217 MpgS/P
-
 Rubrobacter xylanophilus
2.4.1.266 glucosyl-3-phosphoglycerate synthase/phosphatase
-
 Rubrobacter xylanophilus DSM 9941
2.4.1.266 GpgS/P
-
 Rubrobacter xylanophilus DSM 9941

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.217 70 75
-
 Rubrobacter xylanophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.217 additional information
-
 enzyme is thermophilic Rubrobacter xylanophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.217 7 8
-
 Rubrobacter xylanophilus