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Literature summary extracted from

  • Gibson, J.; Geissler, J.F.; Harwood, C.S.
    Benzoate-CoA ligase from Rhodopseudomonas palustris (1990), Methods Enzymol., 188, 154-159.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.0025
-
 ATP
-
 Rhodopseudomonas palustris
6.2.1.25 0.1
-
 CoASH
-
 Rhodopseudomonas palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.25 Mg2+ required Rhodopseudomonas palustris
6.2.1.25 Mn2+ can replace Mg2+ Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.25 60000 62000 HPLC gel filtration Rhodopseudomonas palustris

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25
-
 Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.25 25
-
-
 Rhodopseudomonas palustris

Storage Stability

EC Number Storage Stability Organism
6.2.1.25 in liquid N2, no greater losses of activity after 3-6 months Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-aminobenzoate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 2-fluorobenzoate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + DELTA1-cyclohexenecarboxylate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + DELTA1-cyclohexenecarboxyl-CoA
-
 ?
6.2.1.25 ATP + DELTA3-cyclohexenecarboxylate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + DELTA3-cyclohexenecarboxyl-CoA
-
 ?
6.2.1.25 ATP + picolinate + CoA
-
 Rhodopseudomonas palustris AMP + diphosphate + picolinyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.25 monomer 1 * 60000-62000, denaturing electrophoresis Rhodopseudomonas palustris