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Literature summary extracted from
- Molecular cloning and biochemical characterization of bovine retina calcineurin (2010), Mol. Cell. Biochem., 333, 73-82.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.16 | Calmodulin | dependent on | Bos taurus |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.16 | cDNA sequence determination and analysis, a 10 amino acid, residues ATVEAIEADE, deletion before the autoinhibitory domain is observed in bovine retina CaN A compared to bovine brain CaN A, sequence comparisona, expression of His-tagged isozymes in Escherichia coli strain M13(pREP4) using expression vector pQE9 | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.16 | additional information | the residues at 407-456 also have an inhibitory effect on CaN A phosphatase activity in addition to the previously known autoinhibitory domain at 457-480 | Bos taurus | |
| 3.1.3.16 | Zn2+ | - |
Bos taurus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.16 | Ca2+ | dependent on | Bos taurus | |
| 3.1.3.16 | Fe2+ | activates the eye isozyme, but might inhibit the brain isozyme | Bos taurus | |
| 3.1.3.16 | Mn2+ | slight stimulation | Bos taurus | |
| 3.1.3.16 | Ni2+ | strong activation | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.16 | 18000 | - |
1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE | Bos taurus |
| 3.1.3.16 | 60000 | - |
1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.16 | Bos taurus | P48452 | tissue-specific isozymes in eye and brain | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.16 | recombinant His-tagged isozymes from Escherichia coli strain M13 by nickel affinity chromatography to near homogeneity | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.16 | brain | - |
Bos taurus | - |
| 3.1.3.16 | eye | CaN is present in all eye tissues | Bos taurus | - |
| 3.1.3.16 | retina | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.16 | 4-nitrophenyl phosphate + H2O | - |
Bos taurus | 4-nitrophenol + phosphate | - |
? | |
| 3.1.3.16 | additional information | residues at 97-201 are essential for bovine retina CaN subunit A phosphatase activity | Bos taurus | ? | - |
? | |
| 3.1.3.16 | phosphorylated histone + H2O | - |
Bos taurus | histone + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.16 | heterotrimer | 1 * 60000, subunit A, + 2 * 18000, subunit B, retina isozyme CaN A and B subunits, SDS-PAGE | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.16 | calcineurin | - |
Bos taurus |
| 3.1.3.16 | CaN | - |
Bos taurus |
| 3.1.3.16 | CaN A phosphatase | - |
Bos taurus |
| 3.1.3.16 | More | calcineurin is a member of the Ser/Thr protein phosphatase family | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.16 | 7 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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