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Literature summary extracted from

  • Young, N.; Pearl, D.K.; Van Brocklyn, J.R.
    Sphingosine-1-phosphate regulates glioblastoma cell invasiveness through the urokinase plasminogen activator system and CCN1/Cyr61 (2009), Mol. Cancer Res., 7, 23-32.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.73 additional information uPA needs to be activated proteolytically. Sphingosine 1-phosphate receptor S1P1 overexpression in U-118 MG cells results in potent stimulation of uPA activity regardless of S1P treatment, expression of receptors S1P2 and S1P3 does not affect uPA activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.73 extracellular uPA is secreted from glioblastoma cells as inactive pro-uPA and is converted to an active form at the cell surface where it binds to its receptor uPAR Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.73 plasminogen + H2O Homo sapiens uPA needs to be activated plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.73 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.73 glioblastoma cell
-
 Homo sapiens
-
3.4.21.73 U-118 MG cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.73 plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?
3.4.21.73 plasminogen + H2O uPA needs to be activated Homo sapiens plasmin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.73 uPA
-
 Homo sapiens
3.4.21.73 Urokinase plasminogen activator
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
3.4.21.73 Homo sapiens inhibition of sphingosine kinase blocks basal expression of uPA and uPAR, as well as glioma cell invasion, however overexpression of sphingosine kinase does not augment sphingosine-1-phosphate receptor-mediated enhancement of uPA activity or invasion down
3.4.21.73 Homo sapiens sphingosine-1-phosphate, S1P, induces expression of uPA and of its receptor uPAr in gliobalstoma multiforme cells. S1P1 receptor overexpression leads to the most dramatic induction of the uPA system and of spheroid invasion, even in the absence of added S1P, while expression of receptors S1P2 and S1P3 does not affect uPA expression, overview up

General Information

EC Number General Information Comment Organism
3.4.21.73 malfunction uPA stimulates glioblastoma multiforme cell invasiveness, in sphingosine-1-phosphate-induced invasion using a spheroid invasion assay Homo sapiens
3.4.21.73 additional information sphingosine kinase is necessary for basal activity of the uPA system and glioma cell invasion, while sphingosine 1-phosphate receptor signaling enhances invasion, partially through uPA and CCN1 Homo sapiens