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Literature summary extracted from

  • Stigliano, I.D.; Caramelo, J.J.; Labriola, C.A.; Parodi, A.J.; DAlessio, C.
    Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals (2009), Mol. Biol. Cell, 20, 3974-3984.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.207 additional information the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement Rattus norvegicus
3.2.1.207 additional information the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement Schizosaccharomyces pombe

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.207 cloning of alpha- and beta-subunits and expression in Escherichia coli strains DH5alpha and JA226 Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.207 E457Q site-directed mutagenesis Schizosaccharomyces pombe
3.2.1.207 additional information construction of alpha or beta-subunit deletion mutant strains, disruption of subunit GIIalpha leads to complete loss of enzyme activity, while in the absence of GIIbeta, the catalytic subunit GIIalpha of Schizosaccharomyces pombe folds to an active conformation able to hydrolyze 4-nitrophenyl alpha-D-glucopyranoside, phenotypes, overview Schizosaccharomyces pombe
3.2.1.207 Q408E site-directed mutagenesis Schizosaccharomyces pombe
3.2.1.207 R438K site-directed mutagenesis Schizosaccharomyces pombe
3.2.1.207 Y463F site-directed mutagenesis Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.207 endoplasmic reticulum membrane
-
 Rattus norvegicus 5789
-
3.2.1.207 endoplasmic reticulum membrane
-
 Schizosaccharomyces pombe 5789
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.207 Glc2Man9GlcNAc2 + H2O Rattus norvegicus
-
 GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O Schizosaccharomyces pombe
-
 GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O Rattus norvegicus
-
 Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O Schizosaccharomyces pombe
-
 Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 additional information Rattus norvegicus the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 ?
-
 ?
3.2.1.207 additional information Schizosaccharomyces pombe the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.207 Rattus norvegicus
-
-
-
3.2.1.207 Schizosaccharomyces pombe
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.207 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O the GIIbeta subunit is not required for GIIalpha activity toward the substrate Rattus norvegicus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O the GIIbeta subunit is not required for GIIalpha activity toward the substrate Schizosaccharomyces pombe 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O
-
 Rattus norvegicus GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O
-
 Schizosaccharomyces pombe GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O i.e. G1M9, alpha-mannosidase-treated glycan from jack bean, structure, overview Rattus norvegicus GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O i.e. G1M9, alpha-mannosidase-treated glycan from jack bean, structure, overview Schizosaccharomyces pombe GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O
-
 Rattus norvegicus Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O
-
 Schizosaccharomyces pombe Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O i.e. G2M9, alpha-mannosidase-treated glycan from jack bean, structure, overview Rattus norvegicus Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O i.e. G2M9, alpha-mannosidase-treated glycan from jack bean, structure, overview Schizosaccharomyces pombe Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 additional information the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 Rattus norvegicus ?
-
 ?
3.2.1.207 additional information the enzyme heterodimer is required to efficiently deglucosylate the physiological substrates G2M9 and G1M9 Schizosaccharomyces pombe ?
-
 ?
3.2.1.207 additional information the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement Rattus norvegicus ?
-
 ?
3.2.1.207 additional information the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement Schizosaccharomyces pombe ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.207 heterodimer the enzyme is a heterodimer whose alpha-subunit GIIalpha bears the glycosyl hydrolase active site, whereas its beta-subunit GIIbeta is involved in GIIbeta endoplasmic reticulum retention and folding, but does not efficiently deglucosylate the physiological substrates Glc2Man9GlcNAc2 and Glc1Man9GlcNAc2 Rattus norvegicus
3.2.1.207 heterodimer the enzyme is a heterodimer whose alpha-subunit GIIalpha bears the glycosyl hydrolase active site, whereas its beta-subunit GIIbeta is involved in GIIbeta endoplasmic reticulum retention and folding, but does not efficiently deglucosylate the physiological substrates Glc2Man9GlcNAc2 and GlcMan9GlcNAc2. GIIbeta is required for an efficient in vitro glucose trimming from G2M9 and G1M9, and processing of both middle and innermost glucoses Schizosaccharomyces pombe
3.2.1.207 More the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement Rattus norvegicus
3.2.1.207 More the interaction of the mannose 6-phosphate receptor homologous domain present in GIIbeta with mannoses in the B and/or C arms of the glycans mediates glycan hydrolysis enhancement Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
3.2.1.207 glucosidase II
-
 Rattus norvegicus
3.2.1.207 glucosidase II
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.207 37
-
 assay at Rattus norvegicus
3.2.1.207 37
-
 assay at Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.207 7.2
-
 assay at Rattus norvegicus
3.2.1.207 7.2
-
 assay at Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
3.2.1.207 physiological function glucosidase II plays a key role in glycoprotein biogenesis in the endoplasmic reticulum. It is responsible for the sequential removal of the two innermost glucose residues from the glycan, Glc3Man9GlcNAc2, transferred to Asn residues in proteins. The enzyme participates in the calnexin/calreticulin cycle, it removes the single glucose unit added to folding intermediates and misfolded glycoproteins by the UDP-Glc:glycoprotein glucosyltransferase Rattus norvegicus
3.2.1.207 physiological function glucosidase II plays a key role in glycoprotein biogenesis in the endoplasmic reticulum. It is responsible for the sequential removal of the two innermost glucose residues from the glycan, Glc3Man9GlcNAc2, transferred to Asn residues in proteins. The enzyme participates in the calnexin/calreticulin cycle, it removes the single glucose unit added to folding intermediates and misfolded glycoproteins by the UDP-Glc:glycoprotein glucosyltransferase Schizosaccharomyces pombe