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Literature summary extracted from

  • Nasab, F.P.; Schulz, B.L.; Gamarro, F.; Parodi, A.J.; Aebi, M.
    All in one: Leishmania major STT3 proteins substitute for the whole oligosaccharyltransferase complex in Saccharomyces cerevisiae (2008), Mol. Biol. Cell, 19, 3758-3768.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.18 Lm STT3 paralogues LmSTT3A, LmSTT3B, LmSTT3C, or LmSTT3D are expressed individually in Saccharomyces cerevisiae. Three complemented the yeast stt3 deletion. LmSTT3D expression suppresses deletions of other essential OTase subunits (Wbp1p, Ost1p, Ost2p, and Swp1p). Leishmania major STT3 paralogues are active enzymes that do not incorporate into the yeast OTase complex, but instead form dimers Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.99.18 80000
-
 2 * 80000, SDS-PAGE Leishmania major
2.4.99.18 550000
-
 non-denaturing PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.18 dolichyl diphosphooligosaccharide + [protein]-L-asparagine Leishmania major
-
 dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.18 Leishmania major
-
-
-
2.4.99.18 Saccharomyces cerevisiae P33767 and Q02791 and P39007 and P41543 and P48439 the enzyme consists of nine subunits. Five of them, Wbp1, Swp1, Stt3, Ost1, and Ost2, are essential for viability of the cell, whereas Ost4, Ost5, Ost3, and Ost6 are not essential but are required for maximal OST activity: Wbp1 (P33767 (SwissProt)), Swp1 (Q02795 (SwissProt)), Stt3 (P39007 (SwissProt)), Ost1 (P41543 (SwissProt)), Ost2 (P46964 (SwissProt)), Ost3 (P48439 (SwissProt)), Ost4 (Q99380 (SwissProt)), Ost5, and Ost6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.18 dolichyl diphosphooligosaccharide + [protein]-L-asparagine
-
 Leishmania major dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + [protein]-L-asparagine various LmOTases have different protein substrate affinities when expressed in yeast. Biosynthetic intermediates as well as the complete Glc3Man9GlcNAc2 oligosaccharide are transferred efficiently to protein by the LmSTT3D OTase isoform without any apparent substrate specificity Leishmania major dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.99.18 dimer 2 * 80000, SDS-PAGE Leishmania major

Synonyms

EC Number Synonyms Comment Organism
2.4.99.18 Lm STT3
-
 Leishmania major
2.4.99.18 oligosaccharyltransferase complex
-
 Saccharomyces cerevisiae
2.4.99.18 STT3 protein
-
 Leishmania major