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Literature summary extracted from

  • Hu, F.; Hu, X.; Ma, C.; Zhao, J.; Xu, J.; Yu, X.
    Molecular characterization of a novel Clonorchis sinensis secretory phospholipase A(2) and investigation of its potential contribution to hepatic fibrosis (2009), Mol. Biochem. Parasitol., 167, 127-134.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 PCR product recombined into the pET-28a expression vector with the coding region in frame with the 6 × His tag, expressed in Escherichia coli BL-21 (DE3) Clonorchis sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 3.347
-
 phosphatidylcholine standard PLA2, in 100 mM Tris-HCl buffer, pH 8.5, at 40°C, 50 mM sodium bis(2-ethylhexyl) sulfosuccinate and 0.05mM phenolsulfonphthalein in isooctane synthetic construct
3.1.1.4 4.847
-
 phosphatidylcholine recombinant group III sPLA2, in 100 mM Tris-HCl buffer, pH 8.5, at 40°C, 50 mM sodium bis(2-ethylhexyl) sulfosuccinate and 0.05mM phenolsulfonphthalein in isooctane Clonorchis sinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ dependent on synthetic construct
3.1.1.4 Ca2+ dependent on Clonorchis sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 34000
-
 x * 34000, SDS-PAGE Clonorchis sinensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Clonorchis sinensis C6YBD6
-
-
3.1.1.4 synthetic construct
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 by centrifugation and gel filtration Clonorchis sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 adult
-
 Clonorchis sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 phosphatidylcholine + H2O
-
 synthetic construct 1-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Clonorchis sinensis 1-acylglycerophosphocholine + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 ? x * 34000, SDS-PAGE Clonorchis sinensis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 GIIIsPLA2
-
 Clonorchis sinensis
3.1.1.4 group III sPLA2
-
 Clonorchis sinensis
3.1.1.4 phospholipase A2
-
 synthetic construct
3.1.1.4 PLA2
-
 synthetic construct
3.1.1.4 secretory phospholipase A2
-
 Clonorchis sinensis

General Information

EC Number General Information Comment Organism
3.1.1.4 physiological function group III sPLA2 may play an important role in the initiation and development of hepatic fibrosis. Expression of collagen III is upregulated in LX-2 cells after incubation with the recombinant protein. Group III sPLA2 can bind to specific sites (specific receptor), not lipids, on LX-2 cells membrane. Group III sPLA2 is a component of excretory-secretory products Clonorchis sinensis