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Literature summary extracted from

  • Manabe, S.; Nariya, H.; Miyata, S.; Tanaka, H.; Minami, J.; Suzuki, M.; Taniguchi, Y.; Okabe, A.
    Purification and characterization of a clostripain-like protease from a recombinant Clostridium perfringens culture (2010), Microbiology, 156, 561-569.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.8 overexpressed in Clostridium perfringens strain 13 Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.8 0.852
-
 benzoyl-L-Arg-4-nitroanilide
-
 Clostridium perfringens
3.4.22.8 25.4
-
 Ac-L-Lys-4-nitroanilide
-
 Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.8 38000
-
 38000, 15000 /16000, SDS-PAGE, 38195, 14916 /15374, TOF mass spectrometry, generated on autocatalytic removal of the propeptide and linker undecapeptide from a single precursor peptide (light chains differ in the N-terminal cleavage site) Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.8 Clostridium perfringens
-
-
-
3.4.22.8 Clostridium perfringens 13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.8 cation-exchange chromatography (SP-Sepharose), ultrafiltration Clostridium perfringens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.8 0.172
-
 substrate benzoyl-L-Lys-4-nitroanilide Clostridium perfringens
3.4.22.8 4.28
-
 substrate benzoyl-L-Arg-4-nitroanilide Clostridium perfringens
3.4.22.8 25.45
-
 substrate Ac-L-Lys-4-nitroanilide Clostridium perfringens
3.4.22.8 123.5
-
 substrate benzoyl-L-Arg-4-nitroanilide Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.8 Ac-L-Lys-4-nitroanilide + H2O
-
 Clostridium perfringens N2-acetyl-L-Lys + 4-nitroaniline
-
 ?
3.4.22.8 Ac-L-Lys-4-nitroanilide + H2O
-
 Clostridium perfringens 13 N2-acetyl-L-Lys + 4-nitroaniline
-
 ?
3.4.22.8 benzoyl-L-Arg-4-nitroanilide + H2O
-
 Clostridium perfringens benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.22.8 benzoyl-L-Arg-4-nitroanilide + H2O
-
 Clostridium perfringens 13 benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.22.8 benzoyl-Lys-4-nitroanilide + H2O
-
 Clostridium perfringens benzoyl-Lys + 4-nitroaniline
-
 ?
3.4.22.8 benzoyl-Lys-4-nitroanilide + H2O
-
 Clostridium perfringens 13 benzoyl-Lys + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.8 dimer 38000, 15000 /16000, SDS-PAGE, 38195, 14916 /15374, TOF mass spectrometry, generated on autocatalytic removal of the propeptide and linker undecapeptide from a single precursor peptide (light chains differ in the N-terminal cleavage site) Clostridium perfringens

Synonyms

EC Number Synonyms Comment Organism
3.4.22.8 clostripain-like protease
-
 Clostridium perfringens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.8 22.53
-
 Ac-L-Lys-4-nitroanilide
-
 Clostridium perfringens
3.4.22.8 109.3
-
 benzoyl-L-Arg-4-nitroanilide
-
 Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.8 6.5
-
 substrates: Bz-L-Arg-p-nitroanilide and Ac-L-Lys-4-nitroanilide Clostridium perfringens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.8 0.887
-
 Ac-L-Lys-4-nitroanilide
-
 Clostridium perfringens
3.4.22.8 128.3
-
 benzoyl-L-Arg-4-nitroanilide
-
 Clostridium perfringens