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Literature summary extracted from

  • Krejcík, Z.; Hollemeyer, K.; Smits, T.H.; Cook, A.M.
    Isethionate formation from taurine in Chromohalobacter salexigens: purification of sulfoacetaldehyde reductase (2010), Microbiology, 156, 1547-1555.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.313 4-oxobutyrate 90% substrate inhibition at 20 mM Chromohalobacter salexigens
1.1.1.313 additional information not inhibited by formaldehyde, acetaldehyde, betaine aldehyde, propionaldehyde, DL-glyceraldehyde, phosphonoacetaldehyde, glyoxylate, 2-oxobutyrate, 4-oxobutyrate and 3-sulfopropanaldehyde Chromohalobacter salexigens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.313 0.061
-
 NADPH in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C Chromohalobacter salexigens
1.1.1.313 0.13
-
 2-sulfoacetaldehyde in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C Chromohalobacter salexigens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.313 27000
-
 SDS-PAGE Chromohalobacter salexigens
1.1.1.313 27100
-
 estimated from amino acid sequence Chromohalobacter salexigens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.313 Chromohalobacter salexigens
-
-
-
1.1.1.313 Chromohalobacter salexigens DSM 3043
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.313 Mono Q column chromatography, phenyl Superose gel filtration Chromohalobacter salexigens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.313 culture condition:ammonium-grown cell IsfD is detected at low levels in extracts of ammonium-grown cells Chromohalobacter salexigens
-
1.1.1.313 culture condition:taurine-grown cell IsfD is detected at high levels in extracts of taurine-grown cells Chromohalobacter salexigens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens isethionate + NADP+
-
 ir
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens DSM 3043 isethionate + NADP+
-
 ir
1.1.1.313 4-oxobutyrate + NADPH + H+
-
 Chromohalobacter salexigens ?
-
 ir
1.1.1.313 4-oxobutyrate + NADPH + H+
-
 Chromohalobacter salexigens DSM 3043 ?
-
 ir
1.1.1.313 additional information NADH is not a substrate Chromohalobacter salexigens ?
-
 ?
1.1.1.313 additional information NADH is not a substrate Chromohalobacter salexigens DSM 3043 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.313 isethionate formation, reductase D
-
 Chromohalobacter salexigens
1.1.1.313 IsfD isoform, this enzyme represents the major portion of the IsfD activity described for taurine-grown cells Chromohalobacter salexigens
1.1.1.313 IsfD2 isoform, this enzyme is responsible for the low activity observed in extracts of ammonium-grown cells Chromohalobacter salexigens
1.1.1.313 NADPH-dependent sulfoacetaldehyde reductase
-
 Chromohalobacter salexigens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.313 9
-
 in Tris/H2SO4 buffer Chromohalobacter salexigens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.313 6.5 9 IsfD shows a broad pH optimum from about pH 6.5 to pH 9.5. There is a steep increase in activity from pH 4.5 (negligible activity) and an equally steep decrease above pH 9.5 Chromohalobacter salexigens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.313 9
-
 IsfD is stable in Tris/H2SO4 buffer, pH 9.0 Chromohalobacter salexigens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.313 NADPH highly specific for NADPH. NADH is not a substrate. Chromohalobacter salexigens