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Literature summary extracted from

  • Bastos, I.M.; Motta, F.N.; Charneau, S.; Santana, J.M.; Dubost, L.; Augustyns, K.; Grellier, P.
    Prolyl oligopeptidase of Trypanosoma brucei hydrolyzes native collagen, peptide hormones and is active in the plasma of infected mice (2010), Microbes Infect., 12, 457-66.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.26 DTT in presence of DTT about 6fold higher catalytic efficiency of the enzyme Trypanosoma brucei brucei

Application

EC Number Application Comment Organism
3.4.21.26 medicine involvement in pathogenesis of sleeping sickness suggested Trypanosoma brucei brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.26 expression in Escherichia coli Trypanosoma brucei brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.26 (3S)-N-benzyl-3-[[(2S)-2-(chloroacetyl)pyrrolidin-1-yl]carbonyl]-3,4-dihydroisoquinoline-2(1H)-carboxamide
-
 Trypanosoma brucei brucei
3.4.21.26 AEBSF 1 mM, 12% inhibition Trypanosoma brucei brucei
3.4.21.26 benzamidine 1 mM, 7.2% inhibition Trypanosoma brucei brucei
3.4.21.26 benzyl (2S)-2-([(2S)-2-[(5-phenyl-4,5-dihydro-1,2-oxazol-3-yl)carbonyl]pyrrolidin-1-yl]carbonyl)pyrrolidine-1-carboxylate
-
 Trypanosoma brucei brucei
3.4.21.26 benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate
-
 Trypanosoma brucei brucei
3.4.21.26 benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-4,5-dihydro-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate
-
 Trypanosoma brucei brucei
3.4.21.26 DFP 0.1 mM, 100% inhibition Trypanosoma brucei brucei
3.4.21.26 leupeptin 0.1 mM, 46.2% inhibition Trypanosoma brucei brucei
3.4.21.26 PCMB 0.1 mM, 98% inhibition Trypanosoma brucei brucei
3.4.21.26 PMSF 1 mM, 18% inhibition Trypanosoma brucei brucei
3.4.21.26 TLCK 0.1 mM, 73.2% inhibition Trypanosoma brucei brucei
3.4.21.26 TPCK 0.1 mM, 91.1% inhibition Trypanosoma brucei brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.26 0.01521
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in absence of DTT Trypanosoma brucei brucei
3.4.21.26 0.04413
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in presence of DTT Trypanosoma brucei brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.26 77600
-
 SDS-PAGE Trypanosoma brucei brucei

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.26 Trypanosoma brucei brucei Q6HA27
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.26 procyclic form
-
 Trypanosoma brucei brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.26 beta-endorphin + h2O
-
 Trypanosoma brucei brucei Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro + Leu-Val-Thr-Leu-Phe-Lys-Asn-Ala + Ile-Ile-Lys-Asn-Ala + Tyr-Lys-Lys-Gly-Glu
-
 ?
3.4.21.26 bradykinin + H2O
-
 Trypanosoma brucei brucei Arg-Pro-Pro + Gly-Phe-Ser-Pro + Phe-Arg
-
 ?
3.4.21.26 collagen I + H2O assay at pH 8.0, 37°C Trypanosoma brucei brucei ?
-
 ?
3.4.21.26 GnRH + H2O
-
 Trypanosoma brucei brucei pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro + Gly-NH2
-
 ?
3.4.21.26 N-Suc-Ala-Ala-Ala-7-amido-4-methylcoumarin + H2O assay at pH 7.5, 25°C Trypanosoma brucei brucei N-Suc-Ala-Ala-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.21.26 N-Suc-Gly-Pro-7-amido-4-methylcoumarin + H2O assay at pH 7.5, 25°C Trypanosoma brucei brucei N-Suc-Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.21.26 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin + H2O assay at pH 7.5, 25°C Trypanosoma brucei brucei N-Suc-Gly-Pro-Leu-Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.21.26 neurotensin + H2O
-
 Trypanosoma brucei brucei pGlu-Leu-Tyr-Glu-Asn-Lys-Pro + Arg-Arg-Pro + Tyr-Ile-Leu
-
 ?
3.4.21.26 TRH + H2O
-
 Trypanosoma brucei brucei L-pyroglutamyl-L-histidyl-L-proline + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.26 POP Tb
-
 Trypanosoma brucei brucei
3.4.21.26 prolyl oligopeptidase
-
 Trypanosoma brucei brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.26 25
-
 assay at Trypanosoma brucei brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.26 7.5
-
 assay at Trypanosoma brucei brucei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.26 7.5 8
-
 Trypanosoma brucei brucei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.26 6 9 16% enzyme activity at pH 6.0, 62% enzyme activity at pH 9.0 Trypanosoma brucei brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.21.26 0.00000034
-
-
 Trypanosoma brucei brucei benzyl (2S)-2-([(2S)-2-[(5-phenyl-4,5-dihydro-1,2-oxazol-3-yl)carbonyl]pyrrolidin-1-yl]carbonyl)pyrrolidine-1-carboxylate
3.4.21.26 0.0000014
-
-
 Trypanosoma brucei brucei benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-4,5-dihydro-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate
3.4.21.26 0.000002
-
-
 Trypanosoma brucei brucei (3S)-N-benzyl-3-[[(2S)-2-(chloroacetyl)pyrrolidin-1-yl]carbonyl]-3,4-dihydroisoquinoline-2(1H)-carboxamide
3.4.21.26 0.0000021
-
-
 Trypanosoma brucei brucei benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate

General Information

EC Number General Information Comment Organism
3.4.21.26 physiological function important role in host interactions Trypanosoma brucei brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.26 33
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in absence of DTT Trypanosoma brucei brucei
3.4.21.26 209
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in presence of DTT Trypanosoma brucei brucei