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Literature summary extracted from

  • Do, B.C.; Dang, T.T.; Berrin, J.G.; Haltrich, D.; To, K.A.; Sigoillot, J.C.; Yamabhai, M.
    Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01 (2009), Microb. Cell Fact., 8, 59.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.78 EDTA 236% of initial activity Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Pichia pastoris Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Mn2+ 35% of initial activity Aspergillus niger
3.2.1.78 Sodium dodecyl sulfate 20% of initial activity Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information Km values for konjac glucomannan of low viscosity, locust bean gum galactomannan, carob galactomannan of low viscosity, and 1,4-beta-D-mannan from carob are 0.6 mg/ml, 2.0 mg/ml, 2.2 mg/ml and 1.5 mg/ml, respectively Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 extracellular recombinant enzyme Aspergillus niger
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Cu2+ 110% of initial activity Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 53000
-
 x * 53000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus niger B6V876 stran BK01
-
3.2.1.78 Aspergillus niger BK01 B6V876 stran BK01
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant enzyme Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 1,4-beta-D-mannan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.78 1,4-beta-D-mannan + H2O
-
 Aspergillus niger BK01 ?
-
 ?
3.2.1.78 birch wood xylan + H2O 9.1% of the activity with locust bean gum Aspergillus niger ?
-
 ?
3.2.1.78 birch wood xylan + H2O 9.1% of the activity with locust bean gum Aspergillus niger BK01 ?
-
 ?
3.2.1.78 carob galactomannan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.78 glucomannan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.78 glucomannan + H2O
-
 Aspergillus niger BK01 ?
-
 ?
3.2.1.78 guar gum + H2O 18% of the activity with locust bean gum Aspergillus niger ?
-
 ?
3.2.1.78 guar gum + H2O 18% of the activity with locust bean gum Aspergillus niger BK01 ?
-
 ?
3.2.1.78 locust bean gum galactomannan + H2O
-
 Aspergillus niger mannobiose + glucosyl-mannosyl oligosaccharides main product, with low amounts of mannotriose and higher manno-oligosaccharides ?
3.2.1.78 additional information negligible activity with starch, carboxymethyl cellulose, and alpha-cellulose Aspergillus niger ?
-
 ?
3.2.1.78 additional information negligible activity with starch, carboxymethyl cellulose, and alpha-cellulose Aspergillus niger BK01 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 53000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 80
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 70
-
 half-life 56 h, pH 4.0 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 148
-
 1,4-beta-D-Mannan 70°C Aspergillus niger
3.2.1.78 215
-
 glucomannan 70°C Aspergillus niger
3.2.1.78 292
-
 carob galactomannan 70°C Aspergillus niger
3.2.1.78 330
-
 locust bean gum galactomannan 70°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 4.5 5
-
 Aspergillus niger