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Literature summary extracted from

  • Johnsen, M.G.; Hansen, O.C.; Stougaard, P.
    Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239 (2010), Microb. Cell Fact., 9, 5.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 the enzyme is expressed recombinantly as a periplasmic enzyme in Escherichia coli strain BMS172 Janthinobacterium sp. 4239

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 31000
-
 x * 33000, native enzyme, SDS-PAGE, x * 31000, recombinant enzyme, SDS-PAGE Janthinobacterium sp. 4239
3.2.1.132 33000
-
 x * 33000, native enzyme, SDS-PAGE, x * 31000, recombinant enzyme, SDS-PAGE Janthinobacterium sp. 4239

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 additional information Janthinobacterium sp. 4239 chitosanases hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Janthinobacterium sp. 4239
-
 isolated from a fresh water lake close to Kangerlussuaq in West Greenland
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O hydrolysis of chitosan hexamer, chitosan substrates are 55-80% deacetylated Janthinobacterium sp. 4239 D-glucosamine + ? dimers and trimers ?
3.2.1.132 additional information chitosanases hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues Janthinobacterium sp. 4239 ?
-
 ?
3.2.1.132 additional information the enzyme is specific for chitosan as substrate, no activity with colloidal chitin, insoluble cellulose, CM cellulose, beta-1,3-glucan, xylan, and arabinoxylan Janthinobacterium sp. 4239 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 33000, native enzyme, SDS-PAGE, x * 31000, recombinant enzyme, SDS-PAGE Janthinobacterium sp. 4239

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 More the chitosanase is related to the glycosyl hydrolase family 46 chitosanases Janthinobacterium sp. 4239

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 45
-
-
 Janthinobacterium sp. 4239

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132 10 60 20%, 30%, and 70% of the maximum activity at 60°C, 10°C, and 30°C, respectively, maximal activity at 45°C Janthinobacterium sp. 4239

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 50
-
 the recombinant chitosanase is stable at temperatures up to 50°C above which the enzyme activity decreases rapidly Janthinobacterium sp. 4239
3.2.1.132 70
-
 the recombinant chitosanase shows hardly activity after 30 min Janthinobacterium sp. 4239

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5 7
-
 Janthinobacterium sp. 4239

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4 8 the recombinant enzyme is inactive above pH 8.0 and shows 90% of maximal activity at pH 4.0 Janthinobacterium sp. 4239