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Literature summary extracted from
- Purification and characterisation of a newly isolated stable long-life Tannase produced by F. subglutinans (Wollenweber and Reinking) Nelson et al (2008), J. Pharm. Innov., 3, 142-151.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Tannic acid | maximum enzyme production (9.38 U/ml) is recorded after 96 h of incubation at 35°C, initial pH 5, in submerged culture (200 rpm) utilising 2% (w/v) tannic acid as a sole carbon source | Fusarium subglutinans |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Ba2+ | competitive inhibitor | Fusarium subglutinans | |
| 3.1.1.20 | Co2+ | competitive inhibitor | Fusarium subglutinans | |
| 3.1.1.20 | Cu2+ | competitive inhibitor | Fusarium subglutinans | |
| 3.1.1.20 | Fe3+ | competitive inhibitor | Fusarium subglutinans | |
| 3.1.1.20 | Zn2+ | competitive inhibitor | Fusarium subglutinans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 3.57 | - |
Tannic acid | purified enzyme | Fusarium subglutinans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Ca2+ | - |
Fusarium subglutinans | |
| 3.1.1.20 | K+ | - |
Fusarium subglutinans | |
| 3.1.1.20 | Mg2+ | - |
Fusarium subglutinans | |
| 3.1.1.20 | Mn2+ | - |
Fusarium subglutinans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 150000 | - |
purified enzyme | Fusarium subglutinans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | Fusarium subglutinans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | to electrophoretic homogeneity through two-step column chromatography | Fusarium subglutinans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.20 | -15°C, 3 months | Fusarium subglutinans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | tannic acid + H2O | - |
Fusarium subglutinans | gallic acid + D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | tannase | - |
Fusarium subglutinans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 40 | - |
maximum tannase activity at 40°C | Fusarium subglutinans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 70 | - |
midpoint of thermal inactivation at 70°C after 60 min of exposure | Fusarium subglutinans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 6 | - |
maximum tannase activity at pH 6.0 | Fusarium subglutinans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 6 | 8 | purified enzyme | Fusarium subglutinans |
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