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Literature summary extracted from

  • Jun, H.; Bing, Y.; Keying, Z.; Xuemei, D.; Daiwen, C.
    Sequencing and expression of the xylanase gene 2 from Trichoderma reesei Rut C-30 and characterization of the recombinant enzyme and its activity on xylan (2009), J. Mol. Microbiol. Biotechnol., 17, 101-109.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xyn2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme without N-terminal signal in Escherichia coli Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Km for beta-1,4-xylan from birchwood is 0.15 mg/ml Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 21000
-
 x * 21000, recombinant His-tagged mature enzyme without N-terminal signal sequence, SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Trichoderma reesei
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Trichoderma reesei B2CNY5 Xyn2 or Rut C-30; gene xyn2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant His-tagged Xyn2 1.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 2350
-
 purified recombinant enzyme Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from birchwood Trichoderma reesei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 21000, recombinant His-tagged mature enzyme without N-terminal signal sequence, SDS-PAGE Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-beta-1,4-xylanase2
-
 Trichoderma reesei
3.2.1.8 Rut C-30
-
 Trichoderma reesei
3.2.1.8 xylanase
-
 Trichoderma reesei
3.2.1.8 Xyn2
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
 Trichoderma reesei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 70 30% of maximal activity at 70°C Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 over 85% activity remaining after 30 min Trichoderma reesei
3.2.1.8 60
-
 over 65% activity remaining after 30 min Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 199.7
-
 beta-1,4-xylan pH 5.0, 50°C, birchwood xylan Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
-
 Trichoderma reesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 3 8 activity range, 40% of maximal activity at pH 3.0 and pH 8.0 Trichoderma reesei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 3.5 7.5 purified recombinant enzyme, stable Trichoderma reesei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Trichoderma reesei isoelectric focusing
-
 7.5