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Literature summary extracted from

  • Xue, Y.; Wu, C.; Branford-White, C.; Ning, X.; Nie, H.; Zhu, L.
    Chemical modification of stem bromelain with anhydride groups to enhance its stability and catalytic activity (2010), J. Mol. Catal. B, 63, 188-193.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.32 additional information bromelain activator solution consists of 0.02 M EDTA and 0.05 M L-cysteine, pH 8.0 Ananas comosus

General Stability

EC Number General Stability Organism
3.4.22.32 the pyromellitic anhydride- and poly(maleic anhydride)-modified bromelain does not lose its activity significantly Ananas comosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 SDS for native bromelain, SDS at 4 and 5 mg/ml causes a significant inhibition of 57% and 61%, respectively Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 2.83
-
 casein poly(maleic anhydride)-modified enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 40°C Ananas comosus
3.4.22.32 4.09
-
 casein pyromellitic anhydride-modified enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 40°C Ananas comosus
3.4.22.32 5.26
-
 casein native enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 40°C Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.32 26000
-
 SDS-PAGE Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 casein + H2O
-
 Ananas comosus L-Tyr + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.32 Bromelain
-
 Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 7
-
 native enzyme Ananas comosus