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Literature summary extracted from

  • Han, Y.; Chen, H.
    A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall (2010), J. Mol. Catal. B, 63, 135-140.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.37 degradation in hydrolysis of corn stover hemicellulose, the xylose production increases by 94.9% and 140% when Trichoderma reesei hemicellulase is supplemented with purified beta-xylosidase and crude cell wall proteins of corn stover, respectively Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 2-mercaptoethanol 10 mM, 42% residual activity Zea mays
3.2.1.37 Ag+ 10 mM, 10% residual activity Zea mays
3.2.1.37 Cu2+ 10 mM, 22% residual activity Zea mays
3.2.1.37 EDTA 10 mM, 36% residual activity Zea mays
3.2.1.37 Hg2+ 10 mM, 8% residual activity Zea mays
3.2.1.37 Ni2+ 10 mM, 13% residual activity Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 2.5
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 cell wall
-
 Zea mays 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 K+ 10 mM, 130% of initial activity Zea mays
3.2.1.37 Li+ 10 mM, 147% of initial activity Zea mays
3.2.1.37 Mn2+ 10 mM, 122% of initial activity Zea mays
3.2.1.37 Na+ 10 mM, 126% of initial activity Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 68500
-
 x * 68500, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Zea mays 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 additional information no substrate: xylan, 4-nitrophenyl-beta-D-cellobiose, 4-nitrophenyl-alpha-L-arabinofuranoside, carboxymethycellulose Zea mays ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Zea mays 2 D-xylose
-
 ?
3.2.1.37 xylopentaose + H2O
-
 Zea mays 5 D-xylose
-
 ?
3.2.1.37 xylotetraose + 3 H2O
-
 Zea mays 4 D-xylose
-
 ?
3.2.1.37 xylotriose + H2O
-
 Zea mays 3 D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 68500, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 37
-
-
 Zea mays

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 30 50
-
 Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 45
-
 30 min, 13.6% loss of activity Zea mays
3.2.1.37 50
-
 30 min, loss of 20% of activity Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 6.5
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4.5
-
-
 Zea mays