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Literature summary extracted from

  • Iwai, N.; Tanaka, T.; Kitazume, T.
    Utility of ionic liquid for improvement of fluorination reaction with immobilized fluorinase (2009), J. Mol. Catal. B, 59, 131-133.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.5.1.63 biotechnology the rate of the enzymatic fluorination reaction can be enhanced by using ionic liquids and immobilizing the enzyme with a water-absorbing polymer which stabilizes the enzyme, 1-octyl-3-methylimidazolium hexafluorophosphate and 1-hexyl-3-methylimidazolium hexafluorophosphate raise the conversion yield 2.4times or 1.6times compared to Tris-HCl buffer, pH 8.0, 1-hexyl-3-methylimidazolium tetrafluoroborate, 1-octyl-3-methylimidazolium tetrafluoroborate, 1-octyl-3-methylimidazolium hexafluorophosphate, and 1-hexyl-3-methylimidazolium hexafluorophosphate increase total conversation yields 2.2times to 4times more in immobilized compared to non-immobilized enzyme Streptomyces cattleya

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.63 expression of His-tagged enzyme in Escherichia coli BL21(DE3) pLysS with plasmid pET28b+ Streptomyces cattleya

General Stability

EC Number General Stability Organism
2.5.1.63 water stabilizes the enzyme, thus water-absorbing polymer and ionic liquid is used to immobilize the enzyme and keep it surrounded by water Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya 20 mg water-absorbing polymer in ionic liquid or 50 mM Tris-HCl buffer, pH 8.0, 50 nM S-adenosyl-L-methionine, 4 mircoM KF, 37°C, exteraction of product 5'-deoxy-5'-fluoroadenosine with diethyl ether 5'-deoxy-5'-fluoroadenosine + L-methionine
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.63 nickel-affinity column Streptomyces cattleya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride 20 mg water-absorbing polymer in ionic liquid or 50 mM Tris-HCl buffer, pH 8.0, 50 nM S-adenosyl-L-methionine, 4 mircoM KF, 37°C, exteraction of product 5'-deoxy-5'-fluoroadenosine with diethyl ether Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 adenosyl-fluoride synthase
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 Streptomyces cattleya