EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.208 | expressed in Escherichia coli as an N-terminal GST-fusion protein that is subsequently cleaved with thrombin | Streptomyces viridochromogenes |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.208 | hanging-drop vapour-diffusion method at 20°C. Crystals obtained belong to space group P4(1)2(1)2 and contained two subunits of AviRb per crystallographic asymmetric unit | Streptomyces viridochromogenes |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.208 | E234A | 20% of wild-type activity | Streptomyces viridochromogenes |
2.1.1.208 | E234Q | 70% of wild-type activity | Streptomyces viridochromogenes |
2.1.1.208 | N139A | less than 5% of wild-type activity | Streptomyces viridochromogenes |
2.1.1.208 | N262A | less than 5% of wild-type activity | Streptomyces viridochromogenes |
2.1.1.208 | N262Q | 60% of wild-type activity | Streptomyces viridochromogenes |
2.1.1.208 | R145A | less than 5% of wild-type activity | Streptomyces viridochromogenes |
2.1.1.208 | T135V | 110% of wild-type activity | Streptomyces viridochromogenes |
EC Number | General Stability | Organism |
---|---|---|
2.1.1.208 | AviRb solutions with concentrations above about 0.5 mg/ml are unstable at ionic strengths below 0.3 | Streptomyces viridochromogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.208 | 31602 | - |
2 * 31602, calculated from sequence | Streptomyces viridochromogenes |
2.1.1.208 | 60000 | - |
dynamic light scattering | Streptomyces viridochromogenes |
2.1.1.208 | 67000 | - |
gel filtration | Streptomyces viridochromogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.208 | S-adenosyl-L-methionine + uridine2479 in 23S rRNA | Streptomyces viridochromogenes | AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin | S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.208 | Streptomyces viridochromogenes | Q9F5K6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.208 | - |
Streptomyces viridochromogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.208 | S-adenosyl-L-methionine + uridine2479 in 23S rRNA | - |
Streptomyces viridochromogenes | S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA | - |
? | |
2.1.1.208 | S-adenosyl-L-methionine + uridine2479 in 23S rRNA | AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin | Streptomyces viridochromogenes | S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.208 | dimer | 2 * 31602, calculated from sequence | Streptomyces viridochromogenes |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.208 | AviRb | - |
Streptomyces viridochromogenes |