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Literature summary extracted from

  • Hong, M.R.; Kim, Y.S.; Joo, A.R.; Lee, J.K.; Kim, Y.S.; Oh, D.K.
    Purification and characterization of a thermostable beta-1,3-1,4-glucanase from Laetiporus sulphureus var. miniatus (2009), J. Microbiol. Biotechnol., 19, 818-822.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Ba2+ 8% inhibition at 1 mM Laetiporus sulphureus
3.2.1.73 Cu2+ 14% inhibition at 1 mM Laetiporus sulphureus
3.2.1.73 EDTA 12% inhibition at 1 mM Laetiporus sulphureus
3.2.1.73 Mg2+ 8% inhibition at 1 mM Laetiporus sulphureus
3.2.1.73 Mn2+ 67% inhibition at 1 mM Laetiporus sulphureus
3.2.1.73 additional information no inhibition by Ca2+ and Fe2+ at 1 mM Laetiporus sulphureus
3.2.1.73 Zn2+ 8% inhibition at 1 mM Laetiporus sulphureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 additional information kinetics Laetiporus sulphureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 Co2+ 9% activation at 1 mM Laetiporus sulphureus
3.2.1.73 Ni2+ 19% activation at 1 mM Laetiporus sulphureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 26000
-
 2 * 26000, SDS-PAGE Laetiporus sulphureus
3.2.1.73 52000
-
 gel filtration Laetiporus sulphureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.73 beta-1,3-1,4-glucan + H2O Laetiporus sulphureus from Hordeum vulgare ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Laetiporus sulphureus
-
 var. miniatus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 native enzyme by 16fold ammonium sulfate fractionation, combined cation and anion exchange chromatography, and another anion exchange chromatography Laetiporus sulphureus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.73 additional information the optimum temperature for the fungal growth is 28°C, the fungus does not grow above 42°C Laetiporus sulphureus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 29
-
 purified native enzyme, substrate is Hordeum vulgare beta-1,3-1,4-glucan Laetiporus sulphureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 beta-1,3-1,4-glucan + H2O from Hordeum vulgare Laetiporus sulphureus ?
-
 ?
3.2.1.73 beta-1,3-1,4-glucan + H2O from Hordeum vulgare, best substrate Laetiporus sulphureus ?
-
 ?
3.2.1.73 carboxymethylcellulose + H2O 18% of the activity with beta-1,3-1,4-glucan from Hordeum vulgare Laetiporus sulphureus ?
-
 ?
3.2.1.73 cellulose + H2O 7% of the activity with beta-1,3-1,4-glucan from Hordeum vulgare Laetiporus sulphureus ?
-
 ?
3.2.1.73 lichenan + H2O 85% of the activity with beta-1,3-1,4-glucan from Hordeum vulgare Laetiporus sulphureus ?
-
 ?
3.2.1.73 additional information also active with laminarin, poor activity with Avicel, rice straw, and xylan, no activity with 4-nitrophenyl-beta-D-hexoside derivatives, substrate specificity, overview Laetiporus sulphureus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 dimer 2 * 26000, SDS-PAGE Laetiporus sulphureus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 beta-1,3-1,4-glucanase
-
 Laetiporus sulphureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 75
-
-
 Laetiporus sulphureus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 45 90 at temperatures of 60°C and 85°C, the enzyme activity is 85% and 80% of maximal activity, respectively Laetiporus sulphureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 additional information
-
 thermal inactivation follow first-order kinetics, overview Laetiporus sulphureus
3.2.1.73 65
-
 purified native enzyme, half-life is over 68 days Laetiporus sulphureus
3.2.1.73 70
-
 purified native enzyme, half-life is 125 h Laetiporus sulphureus
3.2.1.73 75
-
 purified native enzyme, half-life is 22 h Laetiporus sulphureus
3.2.1.73 80
-
 purified native enzyme, half-life is 1 h Laetiporus sulphureus
3.2.1.73 85
-
 purified native enzyme, half-life is about 5 min Laetiporus sulphureus
3.2.1.73 90
-
 purified native enzyme, half-life is 80 s Laetiporus sulphureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 5.3
-
 carboxymethylcellulose pH 4.0, 75°C, substrate from Hordeum vulgare Laetiporus sulphureus
3.2.1.73 13.5
-
 beta-1,3-1,4-glucan pH 4.0, 75°C, substrate from Hordeum vulgare Laetiporus sulphureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 4
-
-
 Laetiporus sulphureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 3 5.5 79% and 73% of the maximum activity, respectively, at pH 3.5 and pH 4.5 Laetiporus sulphureus