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Literature summary extracted from

  • Zhou, C.; Xue, Y.; Zhang, Y.; Zeng, Y.; Ma, Y.
    Recombinant expression and characterization of Thermoanaerobacter tengcongensis thermostable alpha-glucosidase with regioselectivity for high yield isomaltooligosaccharides synthesis (2009), J. Microbiol. Biotechnol., 19, 1547-1556.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 expression in Escherichia coli Caldanaerobacter subterraneus subsp. tengcongensis MB4

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Tris
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 1.08
-
 p-nitrophenyl-alpha-D-glucopyranoside
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 4.58
-
 maltotriose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 6.31
-
 maltose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 6.72
-
 maltopentaose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 16.55
-
 maltotetraose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 89000
-
 3 * 89000, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis MB4

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Caldanaerobacter subterraneus subsp. tengcongensis MB4 Q8RDL1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 about 44.7fold after heating treatment, His-tag column affinity chromatography and gel-filtration Caldanaerobacter subterraneus subsp. tengcongensis MB4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 0.326
-
 after purification Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 0.36
-
 p-nitrophenyl-alpha-D-glucopyranoside Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 0.73
-
 substrate dextrin Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 1.09
-
 substrate maltotetraose Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 2.38
-
 substrate maltopentaose Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 2.85
-
 substrate maltotriose Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 3.26
-
 substrate maltose Caldanaerobacter subterraneus subsp. tengcongensis MB4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O assay at 60°C Caldanaerobacter subterraneus subsp. tengcongensis MB4 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 dextrin + H2O assay at 60°C Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
 ?
3.2.1.20 maltopentaose + H2O assay at 60°C Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O assay at 60°C Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
 ?
3.2.1.20 maltotetraose + H2O assay at 60°C Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
 ?
3.2.1.20 maltotriose + H2O assay at 60°C Caldanaerobacter subterraneus subsp. tengcongensis MB4 D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 trimer 3 * 89000, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis MB4

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 TtGluA
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 60
-
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 60
-
 halflife at 60°C, 2 h Caldanaerobacter subterraneus subsp. tengcongensis MB4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 19.92
-
 p-nitrophenyl-alpha-D-glucopyranoside
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 217.2
-
 maltotriose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 253.5
-
 maltose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 316
-
 maltopentaose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 330.1
-
 maltotetraose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5.5
-
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 4.5 7
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.20 18.45
-
 p-nitrophenyl-alpha-D-glucopyranoside
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 19.95
-
 maltotetraose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 40.17
-
 maltose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 47.01
-
 maltopentaose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4
3.2.1.20 47.43
-
 maltotriose
-
 Caldanaerobacter subterraneus subsp. tengcongensis MB4