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Literature summary extracted from

  • Uyama, T.; Morishita, J.; Jin, X.H.; Okamoto, Y.; Tsuboi, K.; Ueda, N.
    The tumor suppressor gene H-Rev107 functions as a novel Ca2+-independent cytosolic phospholipase A1/2 of the thiol hydrolase type (2009), J. Lipid Res., 50, 685-693.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.32 dithiothreitol
-
 Mus musculus
3.1.1.32 dithiothreitol
-
 Homo sapiens
3.1.1.32 dithiothreitol required for full activity. Addition of 2 mM increases PLA1/2 activity 167fold Rattus norvegicus
3.1.1.32 additional information purified H-Rev107 exhibits an 88fold higher PLA1/2 activity than the cytosolic fraction Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.32 cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells Mus musculus
3.1.1.32 cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells Homo sapiens
3.1.1.32 expression vector harboring H-Rev107 with a FLAG tag at the N terminus. Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.32 additional information deletion mutant of H-Rev107 lacking either N-terminal 8 or N-terminal 16 amino acids (DELTAN1, DELTAN2) or C-terminal 25 amino acids (DELTAC1). Expression of the mutants with the expected molecular masses. As for PLA1/2 activity, DELTAN1 shows very low activity (about 3% that of the wild-type), and DELTAN2 and DELTAC1 are entirely inactive Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.32 iodoacetic acid
-
 Homo sapiens
3.1.1.32 iodoacetic acid
-
 Mus musculus
3.1.1.32 iodoacetic acid
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 cytosol
-
 Mus musculus 5829
-
3.1.1.32 cytosol
-
 Homo sapiens 5829
-
3.1.1.32 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.32 additional information H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 Mus musculus
3.1.1.32 additional information H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 Homo sapiens
3.1.1.32 additional information H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.32 19000
-
 x * 19000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Homo sapiens
-
-
-
3.1.1.32 Mus musculus
-
-
-
3.1.1.32 Rattus norvegicus P53817
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32
-
 Mus musculus
3.1.1.32
-
 Homo sapiens
3.1.1.32 FLAG-tagged H-Rev107 by immunoprecipitation Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 brain
-
 Mus musculus
-
3.1.1.32 testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 dipalmitoylphosphatidylcholine + H2O
-
 Mus musculus palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
 ?
3.1.1.32 dipalmitoylphosphatidylcholine + H2O
-
 Homo sapiens palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
 ?
3.1.1.32 dipalmitoylphosphatidylcholine + H2O
-
 Rattus norvegicus palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
 ?
3.1.1.32 additional information His21 and Cys111 of H-Rev107 form a catalytic dyad. The N-terminal proline-rich and C-terminal hydrophobic domains are involved in the catalytic activity and/or proper folding Rattus norvegicus ?
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Mus musculus 2-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Homo sapiens 2-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Rattus norvegicus 2-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylethanolamine + H2O
-
 Mus musculus 2-acylglycerophosphoethanolamine + a carboxylate
-
 ?
3.1.1.32 phosphatidylethanolamine + H2O
-
 Homo sapiens 2-acylglycerophosphoethanolamine + a carboxylate
-
 ?
3.1.1.32 phosphatidylethanolamine + H2O
-
 Rattus norvegicus 2-acylglycerophosphoethanolamine + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.32 ? x * 19000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 H-Rev107
-
 Mus musculus
3.1.1.32 H-Rev107
-
 Homo sapiens
3.1.1.32 H-Rev107
-
 Rattus norvegicus
3.1.1.32 phospholipase A1/2
-
 Mus musculus
3.1.1.32 phospholipase A1/2
-
 Homo sapiens
3.1.1.32 phospholipase A1/2
-
 Rattus norvegicus
3.1.1.32 PLA1/2
-
 Mus musculus
3.1.1.32 PLA1/2
-
 Homo sapiens
3.1.1.32 PLA1/2
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
3.1.1.32 physiological function H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation Mus musculus
3.1.1.32 physiological function H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation Homo sapiens
3.1.1.32 physiological function H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation Rattus norvegicus