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Literature summary extracted from

  • Borgen, B.H.; Thangstad, O.P.; Ahuja, I.; Rossiter, J.T.; Bones, A.M.
    Removing the mustard oil bomb from seeds: transgenic ablation of myrosin cells in oilseed rape (Brassica napus) produces MINELESS seeds (2010), J. Exp. Bot., 61, 1683-1697.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.147 recombinant gene Myr1.Bn1, expressionin transgenic Brassica napus plants Brassica napus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.147 additional information genetic modification of Brassica napus plants to remove myrosinase-storing idioblasts to eliminate release of cell toxic reaction products and metabolites. Construction of transgenic plants ectopically expressing barnase, a ribonuclease, using a seed myrosin cell-specific Myr1.Bn1 promoter, which is lethal for the embryo. Co-expressing barnase under the control of the Myr1.Bn1 promoter with the barnase inhibitor, barstar, under the control of the cauliflower mosaic virus 35S promoter enables a selective and controlled death of myrosin cells without affecting plant viability. Transgenic plants with myrosin defence cells show negligible production of glucosinolate hydrolysis products and altered epithiospecifier protein profile and glucosinolate levels, overview. Glucosinolate profiles of wild-type and mutant plants, overview Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.147 additional information Brassica napus MYR is a beta-thioglucosidase that hydrolyses glucosinolates to a variety of products such as isothiocyanates, thiocyanates, nitriles, epithionitriles, and oxazolidine-thiones depending on the nature of the glucosinolates. Glucosinolates are themselves biologically inactive, but glucosinolates hydrolytic products, such as thiocyanates, isothiocyanates, nitriles, and oxazolidine-2-thione, produced by MYR during processing of oilseed rape meal are biologically active. Glucosinolate profiles of wild-type and mutant plants, overview ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Brassica napus Q9STD7 recombinant gene Myr1.Bn1; cv. Westar, gene Myr1
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 cotyledon
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 Brassica napus
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3.2.1.147 idioblast myrosinase-storing cells, i.e. myrosin cells Brassica napus
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3.2.1.147 seed
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 Brassica napus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 additional information MYR is a beta-thioglucosidase that hydrolyses glucosinolates to a variety of products such as isothiocyanates, thiocyanates, nitriles, epithionitriles, and oxazolidine-thiones depending on the nature of the glucosinolates. Glucosinolates are themselves biologically inactive, but glucosinolates hydrolytic products, such as thiocyanates, isothiocyanates, nitriles, and oxazolidine-2-thione, produced by MYR during processing of oilseed rape meal are biologically active. Glucosinolate profiles of wild-type and mutant plants, overview Brassica napus ?
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 ?
3.2.1.147 sinigrin + H2O i.e. 2-propenyl glucosinolate Brassica napus allyl isothiocyanate + D-glucose + ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.147 MYR
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 Brassica napus
3.2.1.147 myrosinase
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 Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 5.5
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 assay at Brassica napus

General Information

EC Number General Information Comment Organism
3.2.1.147 physiological function myrosinase-catalysed release of toxic and bioactive compounds such as isothiocyanates, upon activation or tissue damage, play a role in the plant defense system Brassica napus