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Literature summary extracted from

  • Eberl, A.; Heumann, S.; Brueckner, T.; Araujo, R.; Cavaco-Paulo, A.; Kaufmann, F.; Kroutil, W.; Guebitz, G.M.
    Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules (2009), J. Biotechnol., 143, 207-212.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.74 additional information no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket Fusarium solani
3.1.1.74 additional information no activation in the presence of Triton X-100 due to the absence of a lid covering the active site pocket Thermobifida fusca
3.1.1.74 N,N-diethyl-2-phenylacetamide increases hydrolysis rates of semi-crystalline poly(ethylene terephthalate) films and fabrics for cutinase. The outer layers of the polymer will be better exposed to the enzyme in the presence of N,N-diethyl-2-phenylacetamide. Overall enhancement in colour depth of 300% Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Fusarium solani
-
 pisi
-
3.1.1.74 Thermobifida fusca
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.74 4°C Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 2-hydroxyethyl benzoate + H2O is hydrolyzed after 24 h of incubation of bisbenzoyloxyethyl terephthalate Fusarium solani ethane-1,2-diol + benzoate
-
 ?
3.1.1.74 2-hydroxyethyl benzoate + H2O is hydrolyzed after 24 h of incubation of bisbenzoyloxyethyl terephthalate Thermobifida fusca ethane-1,2-diol + benzoate
-
 ?
3.1.1.74 bis(2-hydroxyethyl)terephthalate + H2O fast hydrolysis after treatment for 30 min Fusarium solani ?
-
 ?
3.1.1.74 bis(2-hydroxyethyl)terephthalate + H2O hydrolysis after treatment for 5 h Thermobifida fusca ?
-
 ?
3.1.1.74 bisbenzoyloxyethyl terephthalate + H2O
-
 Fusarium solani terephthalic acid + mono(2-hydroxyethyl) terephthalate + bis(2-hydroxyethyl)terephthalate + benzoic acid + 2-hydroxyethyl benzoate
-
 ?
3.1.1.74 bisbenzoyloxyethyl terephthalate + H2O
-
 Thermobifida fusca terephthalic acid + mono(2-hydroxyethyl) terephthalate + bis(2-hydroxyethyl)terephthalate + benzoic acid + 2-hydroxyethyl benzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.74 cutinase
-
 Fusarium solani
3.1.1.74 cutinase
-
 Thermobifida fusca