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Literature summary extracted from

  • Choi, K.H.; Seo, J.Y.; Park, K.M.; Park, C.S.; Cha, J.
    Characterization of glycosyl hydrolase family 3 beta-N-acetylglucosaminidases from Thermotoga maritima and Thermotoga neapolitana (2009), J. Biosci. Bioeng., 108, 455-459.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 gene cbsA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Thermotoga neapolitana
3.2.1.52 gene nagA, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Thermotoga maritima MSB8

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.28
-
 4-nitrophenyl N-acetyl-beta-D-glucosamine pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 0.29
-
 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 0.3
-
 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana
3.2.1.52 0.36
-
 4-nitrophenyl N-acetyl-beta-D-glucosamine pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana
3.2.1.52 0.37
-
 4-nitrophenyl di-N-acetyl-beta-D-chitobiose pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 0.44
-
 4-nitrophenyl di-N-acetyl-beta-D-chitobiose pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 54000
-
 2 * 54000, recombinant enzyme, SDS-PAGE Thermotoga neapolitana
3.2.1.52 54000
-
 2 * 54000, recombinant enzyme, SDS-PAGE Thermotoga maritima MSB8

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Thermotoga maritima MSB8 Q9WZR6 gene nagA
-
3.2.1.52 Thermotoga neapolitana
-
 gene cbsA
-
3.2.1.52 Thermotoga neapolitana KCCM 41025
-
 gene cbsA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration Thermotoga neapolitana
3.2.1.52 recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration Thermotoga maritima MSB8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl di-N-acetyl-beta-D-chitobiose + H2O NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga neapolitana 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl di-N-acetyl-beta-D-chitobiose + H2O NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga maritima MSB8 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl di-N-acetyl-beta-D-chitobiose + H2O NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga neapolitana KCCM 41025 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O CbsA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga neapolitana 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga maritima MSB8 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O CbsA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga neapolitana KCCM 41025 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose + H2O NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga neapolitana 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose + H2O NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga maritima MSB8 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose + H2O NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc Thermotoga neapolitana KCCM 41025 4-nitrophenol + N-acetylglucosamine
-
 ?
3.2.1.52 di-N-acetyl-beta-D-chitobiose + H2O
-
 Thermotoga neapolitana N-acetylglucosamine
-
 ?
3.2.1.52 di-N-acetyl-beta-D-chitobiose + H2O
-
 Thermotoga maritima MSB8 N-acetylglucosamine
-
 ?
3.2.1.52 di-N-acetyl-beta-D-chitobiose + H2O
-
 Thermotoga neapolitana KCCM 41025 N-acetylglucosamine
-
 ?
3.2.1.52 additional information the enzyme shows exo-activity with chitin oligosaccharides from the non-reducing ends, no activity with 4-nitrophenyl-beta-D-galactose and 4-nitrophenyl N-acetyl-beta-D-galactosamine, or highly polymerized chitin Thermotoga neapolitana ?
-
 ?
3.2.1.52 additional information the enzyme shows exo-activity with chitin oligosaccharides from the non-reducing ends, no activity with 4-nitrophenyl-beta-D-galactose and 4-nitrophenyl N-acetyl-beta-D-galactosamine, or highly polymerized chitin Thermotoga maritima MSB8 ?
-
 ?
3.2.1.52 additional information the enzyme shows exo-activity with chitin oligosaccharides from the non-reducing ends, no activity with 4-nitrophenyl-beta-D-galactose and 4-nitrophenyl N-acetyl-beta-D-galactosamine, or highly polymerized chitin Thermotoga neapolitana KCCM 41025 ?
-
 ?
3.2.1.52 tri-N-acetyl-beta-D-chitotriose + H2O
-
 Thermotoga neapolitana N-acetylglucosamine
-
 ?
3.2.1.52 tri-N-acetyl-beta-D-chitotriose + H2O
-
 Thermotoga maritima MSB8 N-acetylglucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer 2 * 54000, recombinant enzyme, SDS-PAGE Thermotoga neapolitana
3.2.1.52 dimer 2 * 54000, recombinant enzyme, SDS-PAGE Thermotoga maritima MSB8

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 beta-N-acetylglucosaminidase
-
 Thermotoga neapolitana
3.2.1.52 beta-N-acetylglucosaminidase
-
 Thermotoga maritima MSB8
3.2.1.52 CbsA
-
 Thermotoga neapolitana
3.2.1.52 More the enzyme belongs to the glycosyl hydrolase family 3, GH3 Thermotoga neapolitana
3.2.1.52 More the enzyme belongs to the glycosyl hydrolase family 3, GH3 Thermotoga maritima MSB8
3.2.1.52 NagA
-
 Thermotoga maritima MSB8

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 65
-
-
 Thermotoga maritima MSB8
3.2.1.52 75
-
-
 Thermotoga neapolitana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 50 95 activity profile, over 65% of maximal activity at 95°C, overview Thermotoga neapolitana
3.2.1.52 50 95 activity profile, over 65% of maximal activity at 95°C, overview Thermotoga maritima MSB8

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 146.7
-
 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 147.2
-
 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana
3.2.1.52 199
-
 4-nitrophenyl di-N-acetyl-beta-D-chitobiose pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 220.8
-
 4-nitrophenyl di-N-acetyl-beta-D-chitobiose pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana
3.2.1.52 246.2
-
 4-nitrophenyl N-acetyl-beta-D-glucosamine pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 273.4
-
 4-nitrophenyl N-acetyl-beta-D-glucosamine pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 7 8
-
 Thermotoga neapolitana
3.2.1.52 7 8
-
 Thermotoga maritima MSB8

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 4.5 10 over 80% of maximal activity within the range of pH 6.5-8.5, rapid decline over pH 9.0, activity profile, overview Thermotoga neapolitana
3.2.1.52 4.5 10 over 80% of maximal activity within the range of pH 6.5-8.5, rapid decline over pH 9.0, activity profile, overview Thermotoga maritima MSB8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.52 490.7
-
 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana
3.2.1.52 501.2
-
 4-nitrophenyl di-N-acetyl-beta-D-chitobiose pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana
3.2.1.52 505.7
-
 4-nitrophenyl tri-N-acetyl-beta-D-chitotriose pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 538
-
 4-nitrophenyl di-N-acetyl-beta-D-chitobiose pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.52 759.4
-
 4-nitrophenyl N-acetyl-beta-D-glucosamine pH 7.5, 70°C, recombinant enzyme Thermotoga neapolitana
3.2.1.52 879.3
-
 4-nitrophenyl N-acetyl-beta-D-glucosamine pH 7.5, 70°C, recombinant enzyme Thermotoga maritima MSB8