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Literature summary extracted from

  • Nakamichi, Y.; Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
    Structural determinants in streptococcal unsaturated glucuronyl hydrolase for recognition of glycosaminoglycan sulfate groups (2011), J. Biol. Chem., 286, 6262-6271.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.179 expression in Escherichia coli Bacillus sp. (in: Bacteria)
3.2.1.180 expression in Escherichia coli Streptococcus agalactiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.180 sitting-drop vapor-diffusion method Streptococcus agalactiae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.179 G342S kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is similar to the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value Bacillus sp. (in: Bacteria)
3.2.1.179 H339S kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.2fold higher than the kcat/Km-value for the wild-type enzyme, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 53% of the wild-type value Bacillus sp. (in: Bacteria)
3.2.1.179 I344K kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 6.1fold higher than the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to the wild-type value Bacillus sp. (in: Bacteria)
3.2.1.180 K370I kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 28% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 5fold higher than wild-type value Streptococcus agalactiae
3.2.1.180 S365H kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.6% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value Streptococcus agalactiae
3.2.1.180 S368G kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 130% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 3.6fold higher than the wild-type value Streptococcus agalactiae
3.2.1.180 T235A kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 2% of the wild-type value Streptococcus agalactiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.179 0.381
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Bacillus sp. (in: Bacteria)
3.2.1.179 0.504
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme G342S Bacillus sp. (in: Bacteria)
3.2.1.179 0.566
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Bacillus sp. (in: Bacteria)
3.2.1.179 0.861
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme H339S Bacillus sp. (in: Bacteria)
3.2.1.179 2.2
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Bacillus sp. (in: Bacteria)
3.2.1.179 7
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme G342S Bacillus sp. (in: Bacteria)
3.2.1.179 7.19
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme H339S Bacillus sp. (in: Bacteria)
3.2.1.179 18.6
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Bacillus sp. (in: Bacteria)
3.2.1.180 0.1
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
3.2.1.180 0.191
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
3.2.1.180 0.371
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
3.2.1.180 0.763
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
3.2.1.180 1.27
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
3.2.1.180 1.4
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
3.2.1.180 1.47
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
3.2.1.180 1.75
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme T235A Streptococcus agalactiae
3.2.1.180 2.36
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.179 Bacillus sp. (in: Bacteria) Q9RC92
-
-
3.2.1.179 Bacillus sp. (in: Bacteria) GL1 Q9RC92
-
-
3.2.1.180 Streptococcus agalactiae Q8E372
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.179
-
 Bacillus sp. (in: Bacteria)
3.2.1.180
-
 Streptococcus agalactiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.179 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O
-
 Bacillus sp. (in: Bacteria) 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.179 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O
-
 Bacillus sp. (in: Bacteria) GL1 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.179 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O kcat/Km is 8% of the kcat/KM value for the unsulfated substrate beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc Bacillus sp. (in: Bacteria) 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.179 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O kcat/Km is 8% of the kcat/KM value for the unsulfated substrate beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc Bacillus sp. (in: Bacteria) GL1 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.180 alpha-D-4-deoxy-DELTA4,5-IdoA-(1->4)-beta-D-GlcNAc + H2O
-
 Streptococcus agalactiae alpha-D-4-deoxy-DELTA4,5-IdoA + beta-D-N-acetylglucosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O kcat/Km is 2% of the kcat/Km-value for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus agalactiae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O
-
 Streptococcus agalactiae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.180 additional information the enzyme acts on unsaturated alpha-iduronic acid as well as unsaturated beta-glucuronic acis residues in substrates, and cleaves both glycoside bonds (1->3) and (1->4) Streptococcus agalactiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.179 UGL
-
 Bacillus sp. (in: Bacteria)
3.2.1.180 UGL ambiguous Streptococcus agalactiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.179 30
-
 assay at Bacillus sp. (in: Bacteria)
3.2.1.180 30
-
 assay at Streptococcus agalactiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.179 14.1
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Bacillus sp. (in: Bacteria)
3.2.1.179 16.9
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme H339S Bacillus sp. (in: Bacteria)
3.2.1.179 18.2
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme G342S Bacillus sp. (in: Bacteria)
3.2.1.179 20.9
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme G342S Bacillus sp. (in: Bacteria)
3.2.1.179 23.8
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Bacillus sp. (in: Bacteria)
3.2.1.179 24.5
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme H339S Bacillus sp. (in: Bacteria)
3.2.1.179 39.8
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Bacillus sp. (in: Bacteria)
3.2.1.179 54.9
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Bacillus sp. (in: Bacteria)
3.2.1.180 1.69
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
3.2.1.180 2.69
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
3.2.1.180 3.85
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
3.2.1.180 3.96
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme T235A Streptococcus agalactiae
3.2.1.180 3.97
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
3.2.1.180 10.2
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
3.2.1.180 10.8
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
3.2.1.180 24.8
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
3.2.1.180 41.6
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.179 7.5
-
 assay at Bacillus sp. (in: Bacteria)
3.2.1.180 7.5
-
 assay at Streptococcus agalactiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.179 2.95
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Bacillus sp. (in: Bacteria)
3.2.1.179 2.99
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme G342S Bacillus sp. (in: Bacteria)
3.2.1.179 3.41
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme H339S Bacillus sp. (in: Bacteria)
3.2.1.179 18.1
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Bacillus sp. (in: Bacteria)
3.2.1.179 19.6
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme H339S Bacillus sp. (in: Bacteria)
3.2.1.179 36.1
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme G342S Bacillus sp. (in: Bacteria)
3.2.1.179 36.8
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Bacillus sp. (in: Bacteria)
3.2.1.179 42
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Bacillus sp. (in: Bacteria)
3.2.1.180 1.63
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
3.2.1.180 2.12
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
3.2.1.180 2.21
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
3.2.1.180 2.26
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme T235A Streptococcus agalactiae
3.2.1.180 7.71
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
3.2.1.180 10.7
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
3.2.1.180 28.2
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
3.2.1.180 102
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
3.2.1.180 130
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae