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Literature summary extracted from

  • Brodkorb, D.; Gottschall, M.; Marmulla, R.; Lüddeke, F.; Harder, J.
    Linalool dehydratase-isomerase, a bifunctional enzyme in the anaerobic degradation of monoterpenes (2010), J. Biol. Chem., 285, 30436-30442.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.127 dithiothreitol the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol Castellaniella defragrans
4.2.1.127 additional information the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool Castellaniella defragrans
5.4.4.4 dithiothreitol the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol Castellaniella defragrans
5.4.4.4 additional information the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool Castellaniella defragrans
5.4.4.4 S-adenosyl-L-methionine 40 mM, 2fold activation Castellaniella defragrans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.127 expression in Escherichia coli Castellaniella defragrans
5.4.4.4 expression in Escherichia coli Castellaniella defragrans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.127 KCl high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely Castellaniella defragrans
4.2.1.127 MgCl2 high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely Castellaniella defragrans
4.2.1.127 additional information not inhibited by 10% (v/v) DMSO. EDTA (5 mM) does not affect the enzyme activity Castellaniella defragrans
4.2.1.127 NaCl high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely Castellaniella defragrans
4.2.1.127 O2 the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool Castellaniella defragrans
4.2.1.127 pyridoxal 5'-phosphate 1 mM; 35% inhibition Castellaniella defragrans
4.2.1.127 S-adenosyl-L-methionine 40 mM, 80% inhibition Castellaniella defragrans
4.2.1.127 Ti(III)citrate 1 mM, complete inhibition Castellaniella defragrans
4.2.1.127 Urea 20% activity remains at 3 M urea, and no activity is detected in 6 M urea Castellaniella defragrans
5.4.4.4 additional information not inhibited by 10% (v/v) DMSO. EDTA (5 mM) does not affect the enzyme activity Castellaniella defragrans
5.4.4.4 O2 the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool Castellaniella defragrans
5.4.4.4 Ti(III)citrate 1 mM, complete inhibition Castellaniella defragrans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.127 0.75
-
 linalool pH 9.0, 35°C Castellaniella defragrans
5.4.4.4 0.5
-
 geraniol pH 9.0, 35°C Castellaniella defragrans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.127 40000
-
 4 * 40000, SDS-PAGE Castellaniella defragrans
4.2.1.127 160000
-
 gel filtration Castellaniella defragrans
5.4.4.4 40000
-
 4 * 40000, SDS-PAGE Castellaniella defragrans
5.4.4.4 160000
-
 gel filtration Castellaniella defragrans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.127 myrcene + H2O Castellaniella defragrans degradation of myrcene linalool
-
 r
4.2.1.127 myrcene + H2O Castellaniella defragrans 65Phen degradation of myrcene linalool
-
 r
5.4.4.4 geraniol Castellaniella defragrans
-
 linalool
-
 r
5.4.4.4 geraniol Castellaniella defragrans 65Phen
-
 linalool
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.127 Castellaniella defragrans E1XUJ2
-
-
4.2.1.127 Castellaniella defragrans 65Phen E1XUJ2
-
-
5.4.4.4 Castellaniella defragrans E1XUJ2
-
-
5.4.4.4 Castellaniella defragrans 65Phen E1XUJ2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.127 proteolytic modification precursor protein with a signal peptide for a periplasmatic location Castellaniella defragrans
5.4.4.4 proteolytic modification precursor protein with a signal peptide for a periplasmic location Castellaniella defragrans

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.127
-
 Castellaniella defragrans
5.4.4.4
-
 Castellaniella defragrans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.127 0.55
-
 pH 9.0, 35°C Castellaniella defragrans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.127 linalool dehydration of linalool to myrcene in the absence of molecular oxygen. The aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyzes in vitro the reaction in both directions depending on the thermodynamic driving forces Castellaniella defragrans myrcene + H2O myrcene is the only product detected r
4.2.1.127 linalool dehydration of linalool to myrcene in the absence of molecular oxygen. The aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyzes in vitro the reaction in both directions depending on the thermodynamic driving forces Castellaniella defragrans 65Phen myrcene + H2O myrcene is the only product detected r
4.2.1.127 additional information neither the monoterpenes alpha- and beta-ocimene nor the monoterpenoids citronellol and nerol are transformed Castellaniella defragrans ?
-
 ?
4.2.1.127 additional information neither the monoterpenes alpha- and beta-ocimene nor the monoterpenoids citronellol and nerol are transformed Castellaniella defragrans 65Phen ?
-
 ?
4.2.1.127 myrcene + H2O degradation of myrcene Castellaniella defragrans linalool
-
 r
4.2.1.127 myrcene + H2O degradation of myrcene Castellaniella defragrans 65Phen linalool
-
 r
5.4.4.4 geraniol
-
 Castellaniella defragrans linalool
-
 r
5.4.4.4 geraniol the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyses in vitro the reaction in both directions depending on the thermodynamic driving forces Castellaniella defragrans linalool
-
 r
5.4.4.4 geraniol
-
 Castellaniella defragrans 65Phen linalool
-
 r
5.4.4.4 geraniol the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyses in vitro the reaction in both directions depending on the thermodynamic driving forces Castellaniella defragrans 65Phen linalool
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.127 tetramer 4 * 40000, SDS-PAGE Castellaniella defragrans
5.4.4.4 tetramer 4 * 40000, SDS-PAGE Castellaniella defragrans

Synonyms

EC Number Synonyms Comment Organism
4.2.1.127 linalool dehydratase-isomerase bifunctional enzyme that catalyzes the reversible dehydration and isomerization of linalool (3,7-dimethyl-1,6-octadien-3-ol) Castellaniella defragrans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.127 35
-
-
 Castellaniella defragrans
5.4.4.4 35
-
 assay at Castellaniella defragrans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.127 28 40 28°C: about 60% of maximal activity, 40°C: about 80% of maximal activity Castellaniella defragrans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.127 9
-
 Tris-HCl buffer, sharp decrease in linalool dehydratase activity beyond pH 9.0 Castellaniella defragrans

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.127 additional information contains no prosthetic group Castellaniella defragrans
5.4.4.4 additional information contains no prosthetic group Castellaniella defragrans