Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Weiner, E.M.; Robson, S.A.; Marohn, M.; Clubb, R.T.
    The sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusual active site architecture (2010), J. Biol. Chem., 285, 23433-23443.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 expressed in Escherichia coli BL21(DE3) cells Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.70 H126A inactive Bacillus anthracis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.70 0.035
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA64 (residues Asp65–Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis
3.4.22.70 0.038
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Bacillus anthracis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 Talon His-affinity resin column chromatography and Sephacryl-100 gel filtration Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 additional information SrtA recognizes the LPXTG-sorting signal through a lock-in-key mechanism Bacillus anthracis ?
-
 ?
3.4.22.70 o-aminobenzoyl-LPETG-2,4-dinitrophenyl + H2O
-
 Bacillus anthracis o-aminobenzoyl-LPETG + 2,4-dinitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 SrtA
-
 Bacillus anthracis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.70 0.0000067
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis
3.4.22.70 0.0000083
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA64 (residues Asp65–Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis

General Information

EC Number General Information Comment Organism
3.4.22.70 physiological function Bacillus anthracis uses the sortase A enzyme to anchor proteins to its cell wall envelope during vegetative growth Bacillus anthracis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.70 0.000183
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis
3.4.22.70 0.000233
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA64 (residues Asp65–Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis