BRENDA - Enzyme Database

Characterization of the human LPIN1-encoded phosphatidate phosphatase isoforms

Han, G.S.; Carman, G.M.; J. Biol. Chem. 285, 14628-14638 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
full-length triple HA-tagged lipin 2 cloned into pCDNA3 expression construct, subcloned into the Ad-track cytomegalovirus vector, and recombined into the Ad-EASY system. Full-length cDNA for lipin 3 from pSPORT-lipin 3 expression vector
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
shRNA
lipin 2 shRNA treatment significantly reduces hepatocyte PAP-1 activity in both wild-type and fld hepatocytes
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytoplasm
majority of lipin 2
Mus musculus
5737
-
3.1.3.4
endoplasmic reticulum membrane
lipin 2
Mus musculus
5789
-
3.1.3.4
lipid droplet
lipin 2
Mus musculus
5811
-
3.1.3.4
nucleus
lipin 2
Mus musculus
5634
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Mus musculus
-
male C57BL/6 mice
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
hepatocyte
lipin 2
Mus musculus
-
3.1.3.4
liver
lipin 2 is enriched in fatty liver dystrophic (fld) mice, whereas lipin 1 and lipin 3 protein is absent
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
709098
Mus musculus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
full-length triple HA-tagged lipin 2 cloned into pCDNA3 expression construct, subcloned into the Ad-track cytomegalovirus vector, and recombined into the Ad-EASY system. Full-length cDNA for lipin 3 from pSPORT-lipin 3 expression vector
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
shRNA
lipin 2 shRNA treatment significantly reduces hepatocyte PAP-1 activity in both wild-type and fld hepatocytes
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytoplasm
majority of lipin 2
Mus musculus
5737
-
3.1.3.4
endoplasmic reticulum membrane
lipin 2
Mus musculus
5789
-
3.1.3.4
lipid droplet
lipin 2
Mus musculus
5811
-
3.1.3.4
nucleus
lipin 2
Mus musculus
5634
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
hepatocyte
lipin 2
Mus musculus
-
3.1.3.4
liver
lipin 2 is enriched in fatty liver dystrophic (fld) mice, whereas lipin 1 and lipin 3 protein is absent
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
709098
Mus musculus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
3.1.3.4
Mus musculus
RNAi against lipin 2 markedly reduces PAP-1 activity in hepatocytes from both wild-type and fld mice and suppresses triglyceride synthesis under conditions of high fatty acid availability
down
3.1.3.4
Mus musculus
lipin 2 protein content is increased in fld liver independent of changes in steady-state Lpin2 mRNA levels. Lipin 2 is dynamically regulated in liver but is not a target gene of peroxisome proliferator-activated receptorgamma-coactivator 1alpha
additional information
3.1.3.4
Mus musculus
both lipin 2 mRNA and protein expression are significantly induced by food deprivation. Lipin 2 knockdown leads to a compensatory increase in Lpin3 mRNA expression, especially in fld hepatocytes
up
General Information
EC Number
General Information
Commentary
Organism
3.1.3.4
malfunction
lipin 1 deficiency does not affect PAP-1 activity in neonatal mice and leads to hepatic triglyceride accumulation. Loss of lipin 2 markedly impairs hepatocyte PAP-1 activity but does not affect basal rates of triglyceride synthesis. Lipin 2 knockdown abrogates triglyceride synthesis under conditions of increased fatty acid availability
Mus musculus
3.1.3.4
physiological function
lipin 2 plays an important role as a hepatic PAP-1 enzyme. Lipin 2 overexpression increases PAP-1 activity in HepG2 cells. Increased hepatic expression of lipin 2 plays a role in increased hepatic triglyceride synthesis rates in ob/ob mice
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.3.4
malfunction
lipin 1 deficiency does not affect PAP-1 activity in neonatal mice and leads to hepatic triglyceride accumulation. Loss of lipin 2 markedly impairs hepatocyte PAP-1 activity but does not affect basal rates of triglyceride synthesis. Lipin 2 knockdown abrogates triglyceride synthesis under conditions of increased fatty acid availability
Mus musculus
3.1.3.4
physiological function
lipin 2 plays an important role as a hepatic PAP-1 enzyme. Lipin 2 overexpression increases PAP-1 activity in HepG2 cells. Increased hepatic expression of lipin 2 plays a role in increased hepatic triglyceride synthesis rates in ob/ob mice
Mus musculus
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.1.3.4
Mus musculus
RNAi against lipin 2 markedly reduces PAP-1 activity in hepatocytes from both wild-type and fld mice and suppresses triglyceride synthesis under conditions of high fatty acid availability
down
3.1.3.4
Mus musculus
lipin 2 protein content is increased in fld liver independent of changes in steady-state Lpin2 mRNA levels. Lipin 2 is dynamically regulated in liver but is not a target gene of peroxisome proliferator-activated receptorgamma-coactivator 1alpha
additional information
3.1.3.4
Mus musculus
both lipin 2 mRNA and protein expression are significantly induced by food deprivation. Lipin 2 knockdown leads to a compensatory increase in Lpin3 mRNA expression, especially in fld hepatocytes
up