EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.4 | V5 epitope-tagged lipin-1 and lipin-2 expression plasmids, transiently transfected into Hepa 16 cells. Wild-type lipin-1A and lipin-2 and mutants lipin-1A-S724L and lipin-2-S731L expressed in HEK-293 cells | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.4 | D679 | mutation of the PAP motif, abolishes PAP activity | Mus musculus |
3.1.3.4 | IL693FF | mutation of the coactivator motif, abolishes PAP activity | Mus musculus |
3.1.3.4 | S724L | lipin-1 mutant | Mus musculus |
3.1.3.4 | S731D | PAP deficiency, does not result from impaired membrane association. Exhibits normal membrane localization | Mus musculus |
3.1.3.4 | S731L | lipin-2 mutant, protein expression at similar levels as the wild-type, thus lack of PAP activity is not related to reduced protein levels or protein stability. PAP deficiency does not result from impaired membrane association. Shows no reduction in membrane association, in multiple trials exhibits a trend toward increased membrane localization compared with wild-type lipin-2, particularly in the absence of oleate. Shows identical coactivator activity to wild-type lipin-1A and lipin-2 | Mus musculus |
3.1.3.4 | S734L | Majeed mutation in lipin-2, which alters a serine residue that is located in the C-LIP domain downstream of the enzyme active site and coactivator motifs. Mutation completely abolishes PAP activity in both lipin-1 and lipin-2 backgrounds | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.4 | cytosol | wild-type and lipin-2 are present at similar levels in cytosolic and membrane fractions in the absence of oleate | Mus musculus | 5829 | - |
3.1.3.4 | membrane | wild-type and lipin-2 are present at similar levels in cytosolic and membrane fractions in the absence of oleate, and it translocates to the membrane fraction with increasing levels of oleate | Mus musculus | 16020 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.4 | Homo sapiens | - |
- |
- |
3.1.3.4 | Mus musculus | - |
C57BL/6J mice | - |
3.1.3.4 | Mus musculus C57/BL6J | - |
C57BL/6J mice | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.4 | blood | lipin-2, in circulating red blood cells, at ca. 10% of the levels observed in liver | Mus musculus | - |
3.1.3.4 | bone | low levels of lipin-2, in bone and bone marrow, at 1020% the levels in liver | Mus musculus | - |
3.1.3.4 | bone marrow | lipin-2 | Mus musculus | - |
3.1.3.4 | brain | lipin-2, in the cortex, hippocampus, thalamus, and hypothalamus | Mus musculus | - |
3.1.3.4 | duodenum | lipin-2 | Mus musculus | - |
3.1.3.4 | esophagus | lipin-2 | Mus musculus | - |
3.1.3.4 | gastrointestinal tract | lipin-2 | Mus musculus | - |
3.1.3.4 | ileum | lipin-2 | Mus musculus | - |
3.1.3.4 | intestinal mucosa | lipin-2, epithelial cell layer | Mus musculus | - |
3.1.3.4 | jejunum | lipin-2 | Mus musculus | - |
3.1.3.4 | kidney | lipin-2, in the cortex, medulla, and papillae | Mus musculus | - |
3.1.3.4 | liver | lipin-2, shows a uniform distribution pattern | Mus musculus | - |
3.1.3.4 | lung | lipin-2 | Mus musculus | - |
3.1.3.4 | salivary gland | lipin-2, submandibular and submaxillary salivary glands | Mus musculus | - |
3.1.3.4 | skin | low levels of lipin-1, whereas lipin-2 is absent | Mus musculus | - |
3.1.3.4 | spleen | lipin-2, in whole spleen and the white blood cell fraction of spleen, at ca. 10% of the levels observed in liver | Mus musculus | - |
3.1.3.4 | thymus | lipin-2 | Mus musculus | - |
3.1.3.4 | tongue | lipin-2, in squamous keratinized epithelium | Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.4 | phosphatidic acid + H2O | - |
Mus musculus | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
3.1.3.4 | phosphatidic acid + H2O | - |
Mus musculus C57/BL6J | 1,2-diacyl-sn-glycerol + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.4 | lipin-1 | - |
Mus musculus |
3.1.3.4 | lipin-1 | - |
Homo sapiens |
3.1.3.4 | lipin-2 | - |
Mus musculus |
3.1.3.4 | lipin-2 | - |
Homo sapiens |
3.1.3.4 | Mg2+-dependent phosphatidate phosphatase | - |
Mus musculus |
3.1.3.4 | Mg2+-dependent phosphatidate phosphatase | - |
Homo sapiens |
3.1.3.4 | PAP | - |
Mus musculus |
3.1.3.4 | PAP | - |
Homo sapiens |
3.1.3.4 | phosphatidate phosphatase | - |
Mus musculus |
3.1.3.4 | phosphatidate phosphatase | - |
Homo sapiens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.1.3.4 | Mus musculus | lipin-2 is most prominently expressed in liver, where levels are much higher than lipin-1, and also in kidney, lung, gastrointestinal tract, and specific regions of the brain. Fasting induces hepatic lipin-1 by 25fold and lipin-2 by 5fold. Lipin-2 is also expressed in circulating red blood cells and sites of lymphopoiesis, like bone marrow, thymus, and spleen | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.4 | malfunction | symptoms of the Majeed syndrome result from a loss of lipin-2 PAP activity. Loss of lipin-2 PAP activity in erythrocytes and lymphocytes may contribute to the anemia and inflammation phenotypes observed in Majeed syndrome patients | Homo sapiens |
3.1.3.4 | physiological function | lipin-2 has transcriptional coactivator activity for peroxisome proliferator-activated receptor-response elements similar to lipin-1. Lipin-1A activates PPRE-luciferase expression ca. 2fold | Mus musculus |