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Literature summary extracted from

  • Donkor, J.; Zhang, P.; Wong, S.; OLoughlin, L.; Dewald, J.; Kok, B.P.; Brindley, D.N.; Reue, K.
    A conserved serine residue is required for the phosphatidate phosphatase activity but not the transcriptional coactivator functions of lipin-1 and lipin-2 (2009), J. Biol. Chem., 284, 29968-29978.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.4 V5 epitope-tagged lipin-1 and lipin-2 expression plasmids, transiently transfected into Hepa 1–6 cells. Wild-type lipin-1A and lipin-2 and mutants lipin-1A-S724L and lipin-2-S731L expressed in HEK-293 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.4 D679 mutation of the PAP motif, abolishes PAP activity Mus musculus
3.1.3.4 IL693FF mutation of the coactivator motif, abolishes PAP activity Mus musculus
3.1.3.4 S724L lipin-1 mutant Mus musculus
3.1.3.4 S731D PAP deficiency, does not result from impaired membrane association. Exhibits normal membrane localization Mus musculus
3.1.3.4 S731L lipin-2 mutant, protein expression at similar levels as the wild-type, thus lack of PAP activity is not related to reduced protein levels or protein stability. PAP deficiency does not result from impaired membrane association. Shows no reduction in membrane association, in multiple trials exhibits a trend toward increased membrane localization compared with wild-type lipin-2, particularly in the absence of oleate. Shows identical coactivator activity to wild-type lipin-1A and lipin-2 Mus musculus
3.1.3.4 S734L Majeed mutation in lipin-2, which alters a serine residue that is located in the C-LIP domain downstream of the enzyme active site and coactivator motifs. Mutation completely abolishes PAP activity in both lipin-1 and lipin-2 backgrounds Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.4 cytosol wild-type and lipin-2 are present at similar levels in cytosolic and membrane fractions in the absence of oleate Mus musculus 5829
-
3.1.3.4 membrane wild-type and lipin-2 are present at similar levels in cytosolic and membrane fractions in the absence of oleate, and it translocates to the membrane fraction with increasing levels of oleate Mus musculus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Homo sapiens
-
-
-
3.1.3.4 Mus musculus
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C57BL/6J mice
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3.1.3.4 Mus musculus C57/BL6J
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C57BL/6J mice
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.4 blood lipin-2, in circulating red blood cells, at ca. 10% of the levels observed in liver Mus musculus
-
3.1.3.4 bone low levels of lipin-2, in bone and bone marrow, at 10–20% the levels in liver Mus musculus
-
3.1.3.4 bone marrow lipin-2 Mus musculus
-
3.1.3.4 brain lipin-2, in the cortex, hippocampus, thalamus, and hypothalamus Mus musculus
-
3.1.3.4 duodenum lipin-2 Mus musculus
-
3.1.3.4 esophagus lipin-2 Mus musculus
-
3.1.3.4 gastrointestinal tract lipin-2 Mus musculus
-
3.1.3.4 ileum lipin-2 Mus musculus
-
3.1.3.4 intestinal mucosa lipin-2, epithelial cell layer Mus musculus
-
3.1.3.4 jejunum lipin-2 Mus musculus
-
3.1.3.4 kidney lipin-2, in the cortex, medulla, and papillae Mus musculus
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3.1.3.4 liver lipin-2, shows a uniform distribution pattern Mus musculus
-
3.1.3.4 lung lipin-2 Mus musculus
-
3.1.3.4 salivary gland lipin-2, submandibular and submaxillary salivary glands Mus musculus
-
3.1.3.4 skin low levels of lipin-1, whereas lipin-2 is absent Mus musculus
-
3.1.3.4 spleen lipin-2, in whole spleen and the white blood cell fraction of spleen, at ca. 10% of the levels observed in liver Mus musculus
-
3.1.3.4 thymus lipin-2 Mus musculus
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3.1.3.4 tongue lipin-2, in squamous keratinized epithelium Mus musculus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 phosphatidic acid + H2O
-
Mus musculus 1,2-diacyl-sn-glycerol + phosphate
-
?
3.1.3.4 phosphatidic acid + H2O
-
Mus musculus C57/BL6J 1,2-diacyl-sn-glycerol + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.4 lipin-1
-
Mus musculus
3.1.3.4 lipin-1
-
Homo sapiens
3.1.3.4 lipin-2
-
Mus musculus
3.1.3.4 lipin-2
-
Homo sapiens
3.1.3.4 Mg2+-dependent phosphatidate phosphatase
-
Mus musculus
3.1.3.4 Mg2+-dependent phosphatidate phosphatase
-
Homo sapiens
3.1.3.4 PAP
-
Mus musculus
3.1.3.4 PAP
-
Homo sapiens
3.1.3.4 phosphatidate phosphatase
-
Mus musculus
3.1.3.4 phosphatidate phosphatase
-
Homo sapiens

Expression

EC Number Organism Comment Expression
3.1.3.4 Mus musculus lipin-2 is most prominently expressed in liver, where levels are much higher than lipin-1, and also in kidney, lung, gastrointestinal tract, and specific regions of the brain. Fasting induces hepatic lipin-1 by 25fold and lipin-2 by 5fold. Lipin-2 is also expressed in circulating red blood cells and sites of lymphopoiesis, like bone marrow, thymus, and spleen up

General Information

EC Number General Information Comment Organism
3.1.3.4 malfunction symptoms of the Majeed syndrome result from a loss of lipin-2 PAP activity. Loss of lipin-2 PAP activity in erythrocytes and lymphocytes may contribute to the anemia and inflammation phenotypes observed in Majeed syndrome patients Homo sapiens
3.1.3.4 physiological function lipin-2 has transcriptional coactivator activity for peroxisome proliferator-activated receptor-response elements similar to lipin-1. Lipin-1A activates PPRE-luciferase expression ca. 2fold Mus musculus