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Literature summary extracted from

  • Heinzelman, P.; Snow, C.D.; Smith, M.A.; Yu, X.; Kannan, A.; Boulware, K.; Villalobos, A.; Govindarajan, S.; Minshull, J.; Arnold, F.H.
    SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability (2009), J. Biol. Chem., 284, 26229-26233.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.91 C313S random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance Phanerodontia chrysosporium
3.2.1.91 C313S random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance Humicola insolens
3.2.1.91 C313S random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance. The C313S mutation increases total Humicola jecorina CBH II activity secreted by the Saccharomyces cerevisiae expression host more than 10fold Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Humicola insolens Q9C1S9
-
-
3.2.1.91 Phanerodontia chrysosporium
-
-
-
3.2.1.91 Trichoderma reesei P07987
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 avicel + H2O microcrystalline cellulose Phanerodontia chrysosporium ?
-
 ?
3.2.1.91 avicel + H2O microcrystalline cellulose Trichoderma reesei ?
-
 ?
3.2.1.91 avicel + H2O microcrystalline cellulose Humicola insolens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CBH II
-
 Phanerodontia chrysosporium
3.2.1.91 CBH II
-
 Trichoderma reesei
3.2.1.91 CBH II
-
 Humicola insolens
3.2.1.91 cellobiohydrolase class II
-
 Phanerodontia chrysosporium
3.2.1.91 cellobiohydrolase class II
-
 Trichoderma reesei
3.2.1.91 cellobiohydrolase class II
-
 Humicola insolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 50
-
 assay at Phanerodontia chrysosporium
3.2.1.91 50
-
 assay at Trichoderma reesei
3.2.1.91 50
-
 assay at Humicola insolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 4.8
-
 assay at Phanerodontia chrysosporium
3.2.1.91 4.8
-
 assay at Trichoderma reesei
3.2.1.91 4.8
-
 assay at Humicola insolens