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Literature summary extracted from

  • Maruyama, Y.; Nakamichi, Y.; Itoh, T.; Mikami, B.; Hashimoto, W.; Murata, K.
    Substrate spcificity of streptococcal unsaturated glucuronyl hydrolases for sulfated glycosaminoglycan (2009), J. Biol. Chem., 284, 18059-18069.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.180 expression in Escherichia coli Streptococcus agalactiae
3.2.1.180 expression in Escherichia coli Streptococcus pneumoniae
3.2.1.180 expression in Escherichia coli Streptococcus pyogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.180 hanging-drop vapor-diffusion method, crystal structure at 1.75 A resolution Streptococcus agalactiae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.180 D115N no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus agalactiae
3.2.1.180 D175N no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus agalactiae
3.2.1.180 R236A effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme Streptococcus agalactiae
3.2.1.180 R236H effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme Streptococcus agalactiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.180 2-mercaptoethanol 1 mM, 20% inhibition Streptococcus pyogenes
3.2.1.180 AlCl3 1 mM, 65% inhibition Streptococcus agalactiae
3.2.1.180 CoCl2 1 mM, 55% inhibition Streptococcus agalactiae
3.2.1.180 D-galactose 5 mM, 21% inhibition Streptococcus pyogenes
3.2.1.180 D-galacturonic acid 5 mM, complete inhibition Streptococcus agalactiae
3.2.1.180 D-galacturonic acid 5 mM, 99% inhibition Streptococcus pneumoniae
3.2.1.180 D-GlcA 5 mM, 97% inhibition Streptococcus pneumoniae
3.2.1.180 D-glucose 5 mM, 40% inhibition Streptococcus pyogenes
3.2.1.180 D-glucuronic acid 5 mM, 98% inhibition Streptococcus agalactiae
3.2.1.180 D-glucuronic acid 5 mM, 98% inhibition Streptococcus pyogenes
3.2.1.180 D-mannose 5 mM, 22% inhibition Streptococcus pyogenes
3.2.1.180 D-xylose 5 mM, 33% inhibition Streptococcus pyogenes
3.2.1.180 KCl 1 mM, 22% inhibition Streptococcus agalactiae
3.2.1.180 L-fucose 5 mM, 50% inhibition Streptococcus pyogenes
3.2.1.180 L-rhamnose 5 mM, 35% inhibition Streptococcus pyogenes
3.2.1.180 LiCl 1 mM, 24% inhibition Streptococcus agalactiae
3.2.1.180 MnCl2 1 mM, 27% inhibition Streptococcus agalactiae
3.2.1.180 N-acetylglucosamine 5 mM, 17% inhibition Streptococcus agalactiae
3.2.1.180 NaCl 1 mM, 22% inhibition Streptococcus agalactiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.180 0.18
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C. wild-type enzyme Streptococcus pneumoniae
3.2.1.180 0.39
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C. wild-type enzyme Streptococcus pyogenes
3.2.1.180 0.54
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C. wild-type enzyme Streptococcus agalactiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.180 43000
-
 1 * 43000, SDS-PAGE Streptococcus agalactiae
3.2.1.180 43000
-
 1 * 43000, SDS-PAGE Streptococcus pneumoniae
3.2.1.180 43000
-
 1 * 43000, SDS-PAGE Streptococcus pyogenes
3.2.1.180 46079
-
 1 * 46079, calculated from sequence Streptococcus pneumoniae
3.2.1.180 46100
-
 1 * 46100, calculated from sequence Streptococcus pyogenes
3.2.1.180 46587
-
 1 * 46587, calculated from sequence Streptococcus agalactiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.180 additional information Streptococcus agalactiae the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans ?
-
 ?
3.2.1.180 additional information Streptococcus pneumoniae the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans ?
-
 ?
3.2.1.180 additional information Streptococcus pyogenes the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans ?
-
 ?
3.2.1.180 additional information Streptococcus pyogenes M1 GAS SF370 the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans ?
-
 ?
3.2.1.180 additional information Streptococcus agalactiae NEM316 the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.180 Streptococcus agalactiae Q8E372
-
-
3.2.1.180 Streptococcus agalactiae NEM316 Q8E372
-
-
3.2.1.180 Streptococcus pneumoniae Q8DR77
-
-
3.2.1.180 Streptococcus pyogenes Q9A0T3
-
-
3.2.1.180 Streptococcus pyogenes M1 GAS SF370 Q9A0T3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.180
-
 Streptococcus agalactiae
3.2.1.180
-
 Streptococcus pneumoniae
3.2.1.180
-
 Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O 10% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus pyogenes 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O 16% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus pneumoniae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O 3.4% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus agalactiae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O 10% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus pyogenes M1 GAS SF370 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O 3.4% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus agalactiae NEM316 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O
-
 Streptococcus agalactiae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O
-
 Streptococcus pneumoniae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O
-
 Streptococcus pyogenes 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O
-
 Streptococcus pyogenes M1 GAS SF370 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O
-
 Streptococcus agalactiae NEM316 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GlcNAc + H2O 1.1% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus pyogenes 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylglucosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GlcNAc + H2O 2% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus pneumoniae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylglucosamine
-
 ?
3.2.1.180 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GlcNAc + H2O 1.1% of the activity with beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus pyogenes M1 GAS SF370 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylglucosamine
-
 ?
3.2.1.180 additional information the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans Streptococcus agalactiae ?
-
 ?
3.2.1.180 additional information the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans Streptococcus pneumoniae ?
-
 ?
3.2.1.180 additional information the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans Streptococcus pyogenes ?
-
 ?
3.2.1.180 additional information shows almost no activity with the unsaturated gellan tetrasaccharide (beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp). Streptococcal enzymes prefer sulfated substrates Streptococcus agalactiae ?
-
 ?
3.2.1.180 additional information shows almost no activity with the unsaturated gellan tetrasaccharide (beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp). Streptococcal enzymes prefer sulfated substrates Streptococcus pneumoniae ?
-
 ?
3.2.1.180 additional information shows almost no activity with the unsaturated gellan tetrasaccharide (beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp). Streptococcal enzymes prefer sulfated substrates Streptococcus pyogenes ?
-
 ?
3.2.1.180 additional information the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans Streptococcus pyogenes M1 GAS SF370 ?
-
 ?
3.2.1.180 additional information shows almost no activity with the unsaturated gellan tetrasaccharide (beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp). Streptococcal enzymes prefer sulfated substrates Streptococcus pyogenes M1 GAS SF370 ?
-
 ?
3.2.1.180 additional information the streptococcal unsaturated glucuronyl hydrolases are capable of degrading unsaturated disaccharides derived from mammalian sulfated glycosaminoglycans. This substrate specificity probably enables Streptococci to readily invade mammalian cells through the degradation of extracellular sulfated glycosaminoglycans Streptococcus agalactiae NEM316 ?
-
 ?
3.2.1.180 additional information shows almost no activity with the unsaturated gellan tetrasaccharide (beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp). Streptococcal enzymes prefer sulfated substrates Streptococcus agalactiae NEM316 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.180 monomer 1 * 43000, SDS-PAGE Streptococcus agalactiae
3.2.1.180 monomer 1 * 43000, SDS-PAGE Streptococcus pneumoniae
3.2.1.180 monomer 1 * 43000, SDS-PAGE Streptococcus pyogenes
3.2.1.180 monomer 1 * 46079, calculated from sequence Streptococcus pneumoniae
3.2.1.180 monomer 1 * 46100, calculated from sequence Streptococcus pyogenes
3.2.1.180 monomer 1 * 46587, calculated from sequence Streptococcus agalactiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.180 SagUGL
-
 Streptococcus agalactiae
3.2.1.180 SpnUGL
-
 Streptococcus pneumoniae
3.2.1.180 SpyUGL
-
 Streptococcus pyogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.180 30
-
 assay at Streptococcus agalactiae
3.2.1.180 30
-
 assay at Streptococcus pneumoniae
3.2.1.180 30
-
 assay at Streptococcus pyogenes
3.2.1.180 37
-
-
 Streptococcus agalactiae
3.2.1.180 37
-
-
 Streptococcus pneumoniae
3.2.1.180 37
-
-
 Streptococcus pyogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.180 45
-
 20 mM Tris-HCl (pH 7.5), 5 min, 50% loss of activity Streptococcus agalactiae
3.2.1.180 45
-
 20 mM Tris-HCl (pH 7.5), 5 min, 50% loss of activity Streptococcus pneumoniae
3.2.1.180 45
-
 20 mM Tris-HCl (pH 7.5), 5 min, 50% loss of activity Streptococcus pyogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.180 1.3
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus pneumoniae
3.2.1.180 4
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus pyogenes
3.2.1.180 24
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.180 5.5
-
 in 20 mM potassium phosphate Streptococcus agalactiae
3.2.1.180 5.5
-
 in 20 mM potassium phosphate Streptococcus pneumoniae
3.2.1.180 5.5
-
 in 20 mM potassium phosphate Streptococcus pyogenes
3.2.1.180 7.5
-
 assay at Streptococcus agalactiae
3.2.1.180 7.5
-
 assay at Streptococcus pneumoniae
3.2.1.180 7.5
-
 assay at Streptococcus pyogenes

Expression

EC Number Organism Comment Expression
3.2.1.180 Streptococcus agalactiae constitutively expressed, expression level increases in presence of glycosaminoglycan up