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Literature summary extracted from

  • Kissen, R.; Bones, A.M.
    Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana (2009), J. Biol. Chem., 284, 12057-12070.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.147 overexpression of nitrile-specifier protein 2, NSP2, in transgenic Arabidopsis thaliana plants leading to increased nitrile production Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.147 Fe2+ shifts the enzyme to nitril fomration with increasing concentration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.147 additional information Arabidopsis thaliana the degradation of glucosinolates is catalyzed by thioglucosidases called myrosinases and leads by default to the formation of isothiocyanates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Arabidopsis thaliana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 2-hydroxy-3-butenylglucosinolate + H2O
-
 Arabidopsis thaliana 5-vinyl-2-oxazolidine thione + D-glucose
-
 ?
3.2.1.147 2-phenylethylglucosinolate + H2O
-
 Arabidopsis thaliana 2-phenylethyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 2-propenylglucosinolate + H2O epithiospecifier protein and nitrile-specifier protein can switch myrosinase-catalyzed degradation of 2-propenylglucosinolate from isothiocyanate to nitrile, only epithiospecifier protein generates the corresponding epithionitrile Arabidopsis thaliana 2-propenyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 3-benzyloxypropylglucosinolate + H2O
-
 Arabidopsis thaliana 3-benzyloxypropyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 3-butenylglucosinolate + H2O
-
 Arabidopsis thaliana 3-butenyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 3-methylthiopropylglucosinolate + H2O
-
 Arabidopsis thaliana 3-methylthiopropyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 4-benzyloxybutylglucosinolate + H2O
-
 Arabidopsis thaliana 4-benzyloxybutyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 4-methylsulfinylbutylglucosinolate + H2O
-
 Arabidopsis thaliana 4-methylsulfinylbutyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 4-methylthiobutylglucosinolate + H2O
-
 Arabidopsis thaliana 4-methylthiobutyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 4-pentenylglucosinolate + H2O
-
 Arabidopsis thaliana 4-pentenyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 5-methylthiopentylglucosinolate + H2O
-
 Arabidopsis thaliana 5-methylthiopentyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 6-methylthiohexylglucosinolate + H2O
-
 Arabidopsis thaliana 6-methylthiohexyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 7-methylthioheptylglucosinolate + H2O
-
 Arabidopsis thaliana 7-methylthioheptyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 8-methylthiooctylglucosinolate + H2O
-
 Arabidopsis thaliana 8-methylthiooctyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 benzylglucosinolate + H2O nitrile-specifier proteins, especially nitrile-specifier protein 2, NSP2, in conjunction with myrosinase enable the enzyme to generate nitriles, overview Arabidopsis thaliana benzylisothiocyanate + D-glucose + ?
-
 ?
3.2.1.147 indolyl-3-methylglucosinolate + H2O
-
 Arabidopsis thaliana indolyl-3-methyl-isothiocyanate + D-glucose
-
 ?
3.2.1.147 additional information the degradation of glucosinolates is catalyzed by thioglucosidases called myrosinases and leads by default to the formation of isothiocyanates Arabidopsis thaliana ?
-
 ?
3.2.1.147 additional information myrosinase acts on glucosinolates to form an unstable aglycone intermediate that can rearrange spontaneously to form an isothiocyanate. Interaction of a protein called epithiospecifier protein with myrosinase diverts the reaction toward the production of epithionitriles or nitriles depending on the glucosinolate structure, while nitrile-specifier proteins, especially nitrile-specifier protein 2, NSP2, enable to generate nitriles in conjunction with myrosinase, tissue distributions of the specifier proteins, overview Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.147 myrosinase
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.2.1.147 malfunction the degradation of glucosinolates is catalyzed by thioglucosidases called myrosinases and leads by default to the formation of isothiocyanates Arabidopsis thaliana