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Literature summary extracted from

  • Saito, T.; Nishi, M.; Lim, M.I.; Wu, B.; Maeda, T.; Hashimoto, H.; Takeuchi, T.; Roos, D.S.; Asai, T.
    A novel GDP-dependent pyruvate kinase isozyme from Toxoplasma gondii localizes to both the apicoplast and the mitochondrion (2008), J. Biol. Chem., 283, 14041-14052.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 additional information no allosteric or regulatory effects are observed in the presence of D-fructose 1,6-diphosphate, D-fructose 2,6-diphosphate, D-glucose 6-phosphate, D-ribose 5-phosphate, AMP, ATP, ITP, AMP, L-His, L-Ser, L-Ala, L-Glu, Gln, L-Thr, L-Met, Gly, L-le, L-Asn, L-Cys, L-Pro, L-Arg, L-Lys, L-Phe, L-Trp, L-Leu, L-Asp, L-Val (1 mM each), L-Tyr (0.5 mM), or 0.1 mM acetyl-CoA Toxoplasma gondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expressed in Escherichia coli BL21(DE3) cells Toxoplasma gondii

General Stability

EC Number General Stability Organism
2.7.1.40 the enzyme activity remains stable after one freeze-thaw cycle Toxoplasma gondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 GDP
-
 Toxoplasma gondii
2.7.1.40 GTP 0.1 mM GTP reduces the Vmax by 10% Toxoplasma gondii
2.7.1.40 IDP
-
 Toxoplasma gondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.0544
-
 GDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2.7.1.40 0.116
-
 phosphoenolpyruvate PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2.7.1.40 0.193
-
 IDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2.7.1.40 7.99
-
 ADP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 apicoplast
-
 Toxoplasma gondii 20011
-
2.7.1.40 mitochondrion
-
 Toxoplasma gondii 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 additional information catalytic activity is independent of K+ Toxoplasma gondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 77000
-
 purified recombinant protein Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Toxoplasma gondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 DEAE-Toyo Pearl 650 column chromatography and glutathione Sepharose column chromatography Toxoplasma gondii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 tachyzoite
-
 Toxoplasma gondii
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 4°C, 20 mM Tris-HCl, pH 7.0, and 30% glycerol, 1 week, remains stable Toxoplasma gondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Toxoplasma gondii ATP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate preference for GDP Toxoplasma gondii GTP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate
-
 Toxoplasma gondii ITP + pyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 PyKII
-
 Toxoplasma gondii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.40 47.6
-
 ADP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2.7.1.40 110
-
 GDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2.7.1.40 131
-
 IDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 8.5
-
-
 Toxoplasma gondii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 8 9.5 more than 80% of the maximal activity is observed between pH 8.0 and pH 9.5 Toxoplasma gondii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.40 7
-
 at pH 7.0, the activity of PyKII is 50% of the maximal activity Toxoplasma gondii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 ADP
-
 Toxoplasma gondii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.40 6
-
 ADP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2.7.1.40 681
-
 IDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii
2.7.1.40 2020
-
 GDP PykII, in 100 mM Tris-HCl,pH 8.5, at 37°C Toxoplasma gondii